M8948

Monoclonal Anti-Myocardin antibody produced in mouse

~2.0 mg/mL, clone MCR54, purified immunoglobulin

• Synonym(e): Anti-MYCD, Anti-MYOCD
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: MCR54, monoclonal
• Application: WB
• Citations: 6

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MCR54, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~150 kDa
• species reactivity: human
• packaging: antibody small pack of 25 μL
• concentration: ~2.0 mg/mL
• technique(s): western blot: 2.5-5 μg/mL using HEK-293T cells overexpressing myocardin
• isotype: IgG2b
• UniProt accession no.: Q8IZQ8
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYOCD(93649)

Beschreibung

General description

Myocardin (MYOCD) is expressed in the muscles and cardiomyocytes. It is a co-transcriptional factor.

Monoclonal Anti-Myocardin (mouse IgG2b isotype) is derived from the hybridoma MCR54 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human myocardin. Myocardin (MYOCD) is a transcriptional coactivator.

Application

Monoclonal Anti-Myocardin antibody produced in mouse has been used in western blot analysisimmunofluorescence.

Biochem/physiol Actions

Myocardin (MYOCD) takes part in the differentiation of vascular smooth muscle cells (VSMC). It is a co-factor of serum response factor (SRF) and regulates the activation of those genes which are dependent on SRF. MYOCD forms a complex with SRF on DNA.

Myocardin is a smooth and cardiac muscle-specific transcriptional coactivator of serum response factor (SRF). It regulates the expression of a set of cardiac and smooth muscle-specific genes. Myocardin was implicated in the pathogenesis of atherosclerosis, Alzheimer′s disease, and malignant transformation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2