MTOX1013

HepaRG^™ Cells AHR (-/-)

human female liver (hepatocarcinoma and hepatitis C tumor)

• UNSPSC Code: 12352207
• NACRES: NA.81
• Biological source: human female liver (hepatocarcinoma and hepatitis C tumor)

Eigenschaften

• Produktname: HepaRG^™ Cells AHR (-/-), one vial, from human female liver(hepatocarcinoma and hepatitis C tumor)
• biological source: human female liver (hepatocarcinoma and hepatitis C tumor)
• Quality Segment: 200
• form: liquid
• storage temp.: −196°C
• Gene Information: human ... AHR(196)

Beschreibung

General description

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG AHR knockout cells express the major xenobiotic sensors (PXR and CAR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

Application

See technical bulletin for detailed protocols

Features and Benefits

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG AHR Knockout (KO) allows conclusive identification of CYP1A1 and CYP1B1 induction through AHR activation.

Zinc finger nucleases (ZFN) mediated Knockout of AHR (AHR) gene.

• The frame-shift mutation of AHR gene was confirmed by fragment length analysis and DNA sequencing.
• Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

Analysis Note

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Legal Information

Exhibit 1: ADME/Tox cell lines license
Exhibit 2: HepaRG limited use license

HepaRG is a trademark of BioPredic International company

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable