The composition of this supplement is considered proprietary and may not be shared. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/806/460/mtoxrcsupdat.pdf
Fortfahren mit
MTOXRCSUP
RPTEC Complete Supplement
sterile-filtered, suitable for cell culture, add to 500 ML of basal medium
Synonym(e):
Basal media supplement for huRPTEC
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
30 mL | Versand in der Regel in 3 Wochen. | € 128,00 |
Über diesen Artikel
NACRES:
NA.81
UNSPSC Code:
12352207
Technischer Dienst
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Unterstützung erhaltenQuality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
storage temp.
−20°C
General description
Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 30 mL of RPTEC complete media supplement which is added to basal media and other components listed below to produce 500 mL of complete media needed for optimal growth, maintenance, and culture of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC). The final RPTEC complete media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).
Application
RPTEC Complete Supplement has been used in SA7K cell line culture for cell characterization.
Preparation Note
Prepare Complete Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-Glutamine (G7513), 30 mL of RPTEC Complete Supplement (MTOXRCSUP), 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Complete Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO2 in air atmosphere.
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Dieser Artikel | |||
|---|---|---|---|
| description sterile-filtered, suitable for cell culture, add to 500 ML of basal medium | description 50 ×, liquid, sterile-filtered, suitable for cell culture | description solution, sterile-filtered, suitable for cell culture | description liquid, sterile-filtered, BioReagent, suitable for cell culture |
| form liquid | form liquid | form solution | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 300 | Quality Level 500 | Quality Level 300 | Quality Level 500 |
Lagerklasse
10 - Combustible liquids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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What does this supplement contain? We are trying optimise media for two populations and need to compare?
1 answer-
Helpful?
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Hello, I was wondering if the FBS in the complete supplement is heat inactivated or not? This would be very helpful for us to understand our recently obtained data. Thank you in advance! With best wishes, Linda
1 answer-
The FBS used to prepare this product has not been heat-inactivated.
Helpful?
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