For optimal solubility, this enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution. Some customers have reported issues with solubility when using other buffers, such as Tris and water. Although an enzymatic assay utilized water, the concentration in that case was very low (0.125 units/ml).
Fortfahren mit
N2415
Nukleosidphosphorylase bacterial
recombinant, expressed in E. coli, ≥10 units/mg protein
Synonym(e):
PNP, Purinnukleosid:Orthophosphatribosyltransferase, Purinnukleosidphosphorylase
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 units | Warenkorb auf Verfügbarkeit prüfen | € 163,00 |
250 units | Warenkorb auf Verfügbarkeit prüfen | € 329,00 |
1000 units | Warenkorb auf Verfügbarkeit prüfen | € 1.010,00 |
Über diesen Artikel
CAS-Nummer:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-857-3
MDL number:
EG-Nummer:
Specific activity:
≥10 units/mg protein
Assay:
≥80% protein basis (biuret)
Recombinant:
expressed in E. coli
€ 163,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenrecombinant
expressed in E. coli
Quality Segment
assay
≥80% protein basis (biuret)
form
lyophilized powder
specific activity
≥10 units/mg protein
sequence note
MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
UniProt accession no.
storage temp.
−20°C
SMILES string
[n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO
InChI
1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1
InChI key
MRWXACSTFXYYMV-FDDDBJFASA-N
Gene Information
Escherichia coli ... deoD(945654)
Application
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.
Nukleosidphosphorylase wird in gekuppelten Enzymsystemen zum Messen der Proteindephosphorylierung verwendet.
Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Biochem/physiol Actions
Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.
Katalysiert die folgende Reaktion:Purinnukleosid + Phosphat = Purin + alpha-D-Ribose-1-Phosphat
Preparation Note
This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.
Other Notes
One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.
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Dieser Artikel | |||
|---|---|---|---|
| description recombinant, expressed in E. coli, ≥10 units/mg protein | description recombinant, expressed in E. coli, ≥1300 U/mL | description - | description Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution |
| assay ≥80% protein basis (biuret) | assay - | assay - | assay - |
| specific activity ≥10 units/mg protein | specific activity ≥1300 U/mL | specific activity ≥25 units/mg protein | specific activity - |
| Gene Information Escherichia coli ... deoD(945654) | Gene Information - | Gene Information - | Gene Information human ... NP(4860) |
| form lyophilized powder | form liquid | form liquid | form buffered aqueous glycerol solution |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. - | UniProt accession no. - |
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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