There has not been any conduct suitability testing for Nile Red. Please see the following for an article that may offer information; J. Histochem. Cytochem. 35:619-621 reference.
To perform the staining, a stock solution of Nile red was prepared by dissolving 10 mg of Nile red in 10 ml of acetone, which was then stored in the refrigerator protected from light. The sections were fixed in 1% glutaraldehyde in PBS for 5 minutes and washed in PBS three times for 2 minutes each. The staining solution was then prepared by diluting 100 ul of the stock solution in 1 ml of PBS. The sections were covered with this solution for 20 minutes at room temperature, then briefly washed in PBS and mounted in 75% glycerol in PBS. Other sections were incubated with solutions containing 50, 10, and 1 ug and 100 ng of Nile red per ml. The sections were examined with a filter set for fluorescein fluorescence (450-500 nm band pass excitation filter, a 510 nm center wavelength chromatic beam splitter, and a 528 nm long-pass barrier filter) and were photographed.
Fortfahren mit
N3013
Nilrot
Powder
Synonym(e):
Nilblau A-Oxazon
Größe auswählen
| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 mg | Warenkorb auf Verfügbarkeit prüfen | € 198,00 |
1 g | Warenkorb auf Verfügbarkeit prüfen | € 1.180,00 |
Über diesen Artikel
Produktname
Nilrot, Technical grade
grade
technical grade
Quality Segment
form
powder
composition
Carbon: 70.0-77.3%
Nitrogen: 7.5-8.8%
technique(s)
titration: suitable
mp
203-205 °C (lit.)
solubility
methanol: 1 mg/mL
λmax
553 nm
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
CCN(CC)c1ccc2N=C3C(Oc2c1)=CC(=O)c4ccccc34
InChI
1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChI key
VOFUROIFQGPCGE-UHFFFAOYSA-N
General description
Application
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description Technical grade | description BioReagent, suitable for fluorescence, ≥97.0% (HPLC) | description suitable for microscopy | description Dye content 95 % |
| technique(s) titration: suitable | technique(s) - | technique(s) - | technique(s) titration: suitable |
| grade technical grade | grade - | grade - | grade - |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) diagnostic assay manufacturing |
| solubility methanol: 1 mg/mL | solubility - | solubility methanol: 0.01 g/10 mL (red to very dark red) | solubility ethanol: 1 mg/mL, clear, dark red |
| form powder | form solid | form crystals | form powder or crystals |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
Still not finding the right product?
Explore all of our products under Nilrot
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
-
How should n3013 be dissolved and diluted for lipid staining?
1 answer-
Helpful?
-