OGS1352

PSF-CMV-PURO-COOH-3C-FRCFP-6HIS - C-TERMINAL 6 HIS AND CFP DUAL TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

• Synonym(e): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• NACRES: NA.85
• UNSPSC Code: 12352200
• Promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: CFP
• Peptide cleavage: 3C

Eigenschaften

• tag: 6-His tagged, cyan fluorescent protein (CFP) tagged
• form: buffered aqueous solution
• mol wt: size 6897 bp
• bacteria selection: kanamycin
• mammalian cells selection: puromycin
• origin of replication: pUC (500 copies)
• peptide cleavage: 3C
• peptide tag location: C-terminal
• promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• reporter gene: CFP
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag: This plasmid contains a c-terminal non-aequorea based cyan/blue fluorescent protein (FrCFP) reporter tag that can be fused to a gene of interest to allow protein detection. This plasmid also contains a secondary Hexa-Histidine (6His) tag protein tag. The sequence of this tag is: HHHHHH. We provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.

About the Cleavage Tag: This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a 3C cleavage tag. The protein sequence of the cleavage tag is: LEVLFQGP. Human Rhinovirus (HRV) 3C Protease is a highly specific protease that cleaves between the Glu and Gly residues of its recognition site. It is often produced with the trademname ′PreScission protease′.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable