OGS1366

PSF-CMV-PURO-COOH-FRCFP - C-TERMINAL CFP TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

• Synonym(e): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• NACRES: NA.85
• UNSPSC Code: 12352200
• Promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: CFP
• Peptide cleavage: no cleavage

Eigenschaften

• tag: cyan fluorescent protein (CFP) tagged
• form: buffered aqueous solution
• mol wt: size 6855 bp
• bacteria selection: kanamycin
• mammalian cells selection: puromycin
• origin of replication: pUC (500 copies)
• peptide cleavage: no cleavage
• peptide tag location: C-terminal
• promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• reporter gene: CFP
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains a c-terminal non-aequorea based cyan/blue fluorescent protein (FrCFP) reporter tag that can be fused to a gene of interest to allow protein detection.

About the Cleavage Tag: This plasmid does not contain a protease cleavage site.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Other Notes

OXGENE′s technology platforms and expert solutions accelerate the discovery, development and manufacture of cell and gene therapies. We work with you to express your gene of interest at scale, with high levels of purity. In addition, our integrated bioinformatics and high-throughput gene editing technology creates a streamlined workflow for automated cell line production. learn more at www.oxgene.com

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable