OGS1367

PSF-CMV-PURO-NH2-KRYFP - N-TERMINAL YFP TAG MAMMALIAN PLASMID

plasmid vector for molecular cloning

• Synonym(e): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• NACRES: NA.85
• UNSPSC Code: 12352200
• Promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: YFP
• Peptide cleavage: no cleavage

Eigenschaften

• tag: yellow fluorescent protein (YFP) tagged
• form: buffered aqueous solution
• mol wt: size 6854 bp
• bacteria selection: kanamycin
• mammalian cells selection: puromycin
• origin of replication: pUC (500 copies)
• peptide cleavage: no cleavage
• peptide tag location: N-terminal
• promoter: Promoter name: CMV; Promoter activity: constitutive; Promoter type: mammalian
• reporter gene: YFP
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains an n-terminal non-aequorea based yellow fluorescent protein (KrYFP) reporter tag that can be fused to a gene of interest to allow protein detection.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable