OGS2891

PSF-OXB20-COOH-TEV-FLAG^®-6HIS - C-TERMINAL 6 HIS AND FLAG^® DUAL TAG BACTERIAL PLASMID

plasmid vector for molecular cloning

• Synonym(e): cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
• UNSPSC Code: 12352200
• Promoter: Promoter name: OXB20; Promoter activity: constitutive; Promoter type: bacterial
• Origin of replication: pUC (500 copies)
• Bacteria selection: kanamycin
• Reporter gene: none
• Peptide cleavage: TEV

Eigenschaften

• recombinant: expressed in E. coli
• tag: 6-His tagged, FLAG^® tagged
• form: buffered aqueous solution
• mol wt: size 3917 bp
• bacteria selection: kanamycin
• origin of replication: pUC (500 copies)
• peptide cleavage: TEV
• peptide tag location: C-terminal
• promoter: Promoter name: OXB20; Promoter activity: constitutive; Promoter type: bacterial
• reporter gene: none
• shipped in: ambient
• storage temp.: −20°C

Beschreibung

General description

PSF-OXB20-COOH-TEV-FLAG^® -6HIS - C-terminal 6 HIS AND FLAG^® dual tag bacterial plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains a c-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK. This plasmid also contains a secondary Hexa-Histidine (6His) tag protein tag. The sequence of this tag is: HHHHHHWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.

About the Cleavage Tag: PSF-OXB20-COOH-TEV-FLAG^®-6HIS - C-terminal 6 HIS AND FLAG^® dual tag bacterial plasmid lso encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a TEV cleavage tag. The protein sequence of the cleavage tag is: ENLYFQG. Cleavage occurs between the Glu and Gly residues. TEV is often reported to have better specificity for its recognition site compared to EKT Thrombin or Faxtor Xa.

Promoter Expression Level: This plasmid vector contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

PSF-OXB20-COOH-TEV-FLAG^® -6HIS - C-terminal 6 HIS AND FLAG^® dual tag bacterial plasmid is a C-terminal 6His and FLAG dual affinity and epitope tag bacterial expression plasmid. This plasmid vector allows the creation of tagged proteins with a TEV cleavage tag.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

Other Notes

To view sequence information for this product, please visit the product page

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable