P6534

Phospholipase A₂ aus Schweinepankreas

ammonium sulfate suspension, ≥600 units/mg protein

• Synonym(e): Lecithinase A, PLA₂
• CAS-Nummer: 9001-84-7
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 232-637-7
• MDL number: MFCD00131890
• EG-Nummer: 3.1.1.4 (BRENDA, IUBMB)
• Specific activity: ≥600 units/mg protein

Eigenschaften

• form: ammonium sulfate suspension
• Quality Segment: 200
• specific activity: ≥600 units/mg protein
• UniProt accession no.: P00592
• storage temp.: 2-8°C
• Gene Information: pig ... PLA2G1B(445525)

Beschreibung

General description

Phospholipase A2 is a small disulfide-rich protein having 124 residues. It is a calcium-dependent enzyme.

Application

Phospholipase A2 has been used in phospholipase assay and to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation.

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

It has a high catalytic activity on aggregated substrates compared to monomeric substrates.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄-Lösung, pH 5.5

Analysis Note

Proteingehalt mittels Biuret-Reaktion bestimmt.

Other Notes

1 U hydrolysiert 1.0 μmol Soja L-α-Phosphatidylcholin zu L-α-Lysophosphatidylcholin und Fettsäure pro Minute bei pH 8.0 und 37 °C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable