This product is soluble in water at 1 mg/mL. For assay purposes, the material is prepared in 100 mM Potassium Phosphate Buffer, pH 7.6. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/245/514/p9136pis.pdf
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P9136
Pyruvat-Kinase aus Kaninchenmuskel
Type III, lyophilized powder, 350-600 units/mg protein
Synonym(e):
ATP:pyruvate 2-O-phosphotransferase, PK, Phospho-enolpyruvat-Kinase
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1000 units | Warenkorb auf Verfügbarkeit prüfen | € 68,90 |
5000 units | Warenkorb auf Verfügbarkeit prüfen | € 170,00 |
25000 units | Warenkorb auf Verfügbarkeit prüfen | € 514,00 |
50000 units | Warenkorb auf Verfügbarkeit prüfen | € 926,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-616-2
NACRES:
NA.54
MDL number:
Specific activity:
350-600 units/mg protein
Biological source:
rabbit muscle
Technischer Dienst
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Unterstützung erhaltenbiological source
rabbit muscle
Quality Segment
type
Type III
form
lyophilized powder
specific activity
350-600 units/mg protein
mol wt
237 kDa
foreign activity
lactic dehydrogenase, creatine phosphokinase, phosphoglucomutase, and myokinase ≤0.01%
storage temp.
−20°C
General description
Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells.
Application
Pyruvate kinase has been used in plant spectrophotometric assays to measure ATP hydrolysis . Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency .
Pyruvate Kinase from rabbit muscle has been used to convert adenosine diphosphate (ADP) to adenosine triphosphate (ATP) in Pseudomonas putida cells.
Biochem/physiol Actions
Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Pyruvate kinase (PK) catalyzes an important process of transferring phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP). This reaction results in the conversion of PEP to pyruvate and adenosine triphosphate (ATP). Pyruvate kinase plays a major role in glycolysis and gluconeogenesis. High levels of pyruvate kinase M2 (PKM2) inhibits cell proliferation and tumor growth. Deficiency of PK in red blood cells (RBC) leads to non-spherocytic hemolytic anaemia. In human erythrocytes, deficiency of PK induces a protective effect against Plasmodium falciparum.
Analysis Note
Protein determined by biuret.
Other Notes
One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description Type III, lyophilized powder, 350-600 units/mg protein | description Type VII, buffered aqueous glycerol solution, 350-600 units/mg protein | description lyophilized powder, ≥20 units/mg protein, 2× crystallization | description ammonium sulfate suspension, ≥100 units/mg protein |
| specific activity 350-600 units/mg protein | specific activity 350-600 units/mg protein | specific activity ≥20 units/mg protein | specific activity ≥100 units/mg protein |
| biological source rabbit muscle | biological source - | biological source rabbit muscle | biological source rabbit muscle |
| form lyophilized powder | form buffered aqueous glycerol solution | form lyophilized powder | form ammonium sulfate suspension |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 237 kDa | mol wt 237 kDa | mol wt 97,200 Da by calculation | mol wt - |
| Quality Level 200 | Quality Level 300 | Quality Level 300 | Quality Level 300 |
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Lagerklasse
11 - Combustible Solids
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flash_point_c
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What buffer/solvent should I use to dissolve?
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What is the sequence for that protein or the PDB/ uniprot link ? Thank you
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The sequence for this protein can be found at https://www.uniprot.org/uniprotkb/P11974.
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