S7901

Stem Cell Factor human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, powder, cell culture | mammalian: suitable

• Synonym(e): SCF, c-Kit ligand
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00165757
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: powder
• Mol wt: monomer 18.6 kDa ((165 aa))
• Impurities: ≤1 EU/μgtested (LAL test)

Eigenschaften

• Produktname: Stem Cell Factor human, SCF, recombinant, expressed in E. coli, powder, suitable for cell culture
• biological source: human
• Quality Segment: 200
• recombinant: expressed in E. coli
• assay: ≥95% (SDS-PAGE)
• form: powder
• potency: ≤15 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: monomer 18.6 kDa ((165 aa))
• packaging: pkg of 10 μg, pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μgtested (LAL test)
• UniProt accession no.: P21583
• storage temp.: −20°C

Beschreibung

General description

Recombinant human Stem Cell Factor (SCF), also known as c‑Kit ligand, is expressed in E. coli and supplied as a lyophilized powder for use in mammalian cell culture research in vitro.

Application

Stem Cell Factor human has been used in

• In vitro differentiation of equine embryonic stem cells
• Chemotaxis detection system
• Ex vivo expansion of umbilical cord blood CD34⁺ cells
• Cytokine therapy

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), has broad effect on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. In the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Physical form

Lyophilized from a 0.2 μm filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Analysis Note

The proliferative activity is tested in culture using TF1 human erythroleukemic cells.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)