It was suggested that to deprotonate L-012 by one-electron oxidation to its radical form, the presence of strong oxidants, in addition to H2O2, is highly recommended. Therefore, the inclusion of HRP is also highly recommended. Moreover, it was mentioned that the presence of superoxide ion alone may not be sufficient to drive the reaction forward. In the event that no reaction was detected, it is highly recommended to titrate up the concentration of H2O2 and HRP, as well as include xanthine oxidase and hypoxanthine to help drive this reaction.
Fortfahren mit
SML2236
L-012 sodium salt
≥98% (HPLC), powder, neurotoxin
Synonym(e):
8-Amino-5-chloro-2,3-dihydro-7-phenyl-Pyrido[3,4-d]pyridazine-1,4-dione, sodium salt, 8-Amino-5-chloro-7-phenyl-2,3-dihydro-pyrido[3,4-d]pyridazine-1,4-dione, sodium salt, L012
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 mg | Warenkorb auf Verfügbarkeit prüfen | € 117,00 |
25 mg | Warenkorb auf Verfügbarkeit prüfen | € 470,00 |
Über diesen Artikel
Empirische Formel (Hill-System):
C13H8ClN4O2 · Na
CAS-Nummer:
Molekulargewicht:
310.67
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
Assay:
≥98% (HPLC)
Form:
powder
Technischer Dienst
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Unterstützung erhaltenProduktname
L-012 sodium salt, ≥98% (HPLC)
assay
≥98% (HPLC)
form
powder
color
yellow to orange
solubility
H2O: 2 mg/mL, clear
storage temp.
−20°C
SMILES string
[Na+].Clc1nc(c(c3c1c(n[nH][c]3=O)[O-])N)c2ccccc2
InChI
1S/C13H9ClN4O2.Na/c14-11-8-7(12(19)17-18-13(8)20)9(15)10(16-11)6-4-2-1-3-5-6;/h1-5H,15H2,(H,17,19)(H,18,20);/q;+1/p-1
InChI key
IGEUYSJHQQCEFP-UHFFFAOYSA-M
Application
Biochem/physiol Actions
L-012 is a luminol analog and a widely used reactive oxygen and nitrogen species (RONS; ROS & RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo. L-012 displays significantly higher CL yield and sensitivity than luminol, lucigenin and MCLA. Instead of reacting with superoxide anion (O2−) directly, L-012 (LumH2) is converted via a one-electron oxidation (catalyzed by peroxidase in the presence of H2O2) to the LumH· radical form, which then reacts with oxygen (O2) to yield O2− and L-012 quinone (q-Lum). The O2− in turn reacts with the LumH· radical, leading eventually to an endoperoxide that decomposes to emit luminescence. To a less extend, H2O2 can also react with q-Lum to yield luminescence. Although not specific to NADPH oxidase-derived RONS, L-012 is also commonly used for screening NADPH oxidase inhibitors.
Widely used reactive oxygen and nitrogen species (RONS; ROS and RNS) chemiluminescence (CL) probe both in cultures and in animals in vivo.
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Dieser Artikel | |||
|---|---|---|---|
| description ≥98% (HPLC) | description ≥98% (HPLC) | description ≥98% (HPLC) | description European Pharmacopoeia (EP) Reference Standard |
| form powder | form powder | form powder | form - |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| solubility H2O: 2 mg/mL, clear | solubility DMSO: 10 mg/mL, clear | solubility DMSO: 25 mg/mL, clear | solubility - |
| color yellow to orange | color white to beige | color white to beige | color - |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Are there any known issues with using SML2236 (L-012 luminol analog) for the detection of reactive oxygen species according to published protocols?
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