This product is a ready-made solution in dimethyl sulfoxide at a concentration of 10 mM. This product can be diluted directly in a suitable medium to achieve a working concentration of 1–10 μM. An intermediate stock solution is not required.
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SML2959
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Ready Made Solution
≥98% (HPLC), liquid, mithophagy inducer
Synonym(e):
FCCP, Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 mL | Warenkorb auf Verfügbarkeit prüfen | € 69,30 |
Über diesen Artikel
Empirische Formel (Hill-System):
C10H5F3N4O
Molekulargewicht:
254.17
UNSPSC Code:
12352200
NACRES:
NA.77
Assay:
≥98% (HPLC)
Form:
liquid
Technischer Dienst
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Unterstützung erhaltenProduktname
Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone Ready Made Solution, ≥98% (HPLC), 2.54 mg/mL (10mM) in DMSO
Quality Segment
assay
≥98% (HPLC)
form
liquid
storage temp.
−20°C
General description
Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP) is a proton (H+) ionophore and a potent mitochondrial uncoupling agent. This compound turns the mitochondrial membrane permeable to protons, thus dissipating the mitochondrial membrane potential and uncouples oxidative phosphorylation from ATP synthesis. FCCP is a potent tool for assaying mitochondrial respiration and investigating the role of mitochondria in cellular function.1,2FCCP induces mitophagy of SH-SY5Y neuroblastoma cell in a dose dependent manner. Low concentration of FCCP (1μM) leads to a complete loss of mitochondrial membrane potential without triggering mitophagy, whereas at a high concentration (10 μM), cytosol acidification is increased and mitochondrial degradation is attained.3 Additionally, FCCP (20 μM) was able to induce apoptosis in lymphoid (CCRF-CEM) and myeloid (HL60) cells after 24hrs of incubation. 4 FCCP (0.5nM-5 μM) reduces β-amyloid peptide production in K695sw cells without altering the β-amyloid precursor protein maturation or synthesis.5 Partial uncoupling of mitochondrial oxidative phosphorylation induced by FCCP (0.3 -3μM) reduces micromotion and wound healing migration of human mesenchymal stem cells.6 Inhibition of F1F0-ATPase by FCCP (10 μM), reduces the impairment of electromechanical activity in human myocardium.7
Biochem/physiol Actions
Mithophagy inducer, ionophore
Other Notes
FCCP ready made solution is a DMSO based solution at 10mM concentration. It is recommended to further dilute in a suitable media to achieve a working concentration of 1-10 μM.
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Dieser Artikel | |||
|---|---|---|---|
| description ≥98% (HPLC), 2.54 mg/mL (10mM) in DMSO | description ≥98% (HPLC), powder | description ≥98% (HPLC), 7.5mg/ml (50mM) in MES buffer, Autophagy inhibitor | description organic cyanylating reagent, 100 mg/mL in acetonitrile |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥97% |
| form liquid | form powder | form liquid | form liquid |
| Quality Level 100 | Quality Level 300 | Quality Level - | Quality Level - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
Lagerklasse
10 - Combustible liquids
Flammpunkt (°F)
188.6 °F
Flammpunkt (°C)
87 °C
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Does this product need to be mixed with DMSO as an intermediate stock first or can it be mixed directly with a medium?
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How stable is this product with respect to freeze/thaw cycles?
1 answer-
The effect of multiple freeze/thaw cycles has not been investigated. This product is prepared in DMSO, which is considered to be a cryoprotectant. For concerns regarding repeated freeze/thaw stability, the product may be aliquoted into single use volumes and stored, undiluted, at -20°C.
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