SML3052

ppTG21 trifluoroacetate salt

≥95% (HPLC)

• Synonym(e): GLFHALLHLLHSLWHLLLHA-OH TFA salt, Gly-Leu-Phe-His-Ala-Leu-Leu-His-Leu-Leu-His-Ser-Leu-Trp-His-Leu-Leu-Leu-His-Ala TFA salt
• Empirische Formel (Hill-System): C₁₁₅H₁₇₃N₃₁O₂₂ · xC₂HF₃O₂
• Molekulargewicht: 2341.80 (free base basis)
• NACRES: NA.77
• UNSPSC Code: 12352200
• Assay: ≥95% (HPLC)
• Form: film or powder

Eigenschaften

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: film or powder
• color: white to beige
• storage temp.: −20°C

Beschreibung

Biochem/physiol Actions

Endosomolytic agent that facilitates cell type-specific gene editing by enabling Cas9 RNP endosome escape following receptor-mediated endocytosis.

ppTG21 is an endosomolytic agent (pI 7.7, charge +1.3 @ pH 7.4) that facilitates cell type-specific gene editing by enabling endosome escape of Cas9 ribonucleoprotein (RNP) complex following its uptake via receptor-mediated endocytosis. Following liver-specific asialoglycoprotein receptor (ASGPr)-mediated delivery of ASGPr ligand (ASGPrL)-harboring & SV40 nuclear localization sequence (NLS)-bearing S. pyogenes Cas9 (Cas9-2lig-1NLS) RNP with EMX1-targeting sgRNA, target gene editing occurs only upon coincubation of the RNP in the presence of 30 molar equiv of ppTG21 (ave 4.8% indels in HepG2 cells vs. 0.2% indels without ppTG21).

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable