SML4170

Desthiobiotin-iodoacetamide

≥98% (HPLC), powder, pan-cysteine profiling tag

• Synonym(e): (4R,5S)-N-[2-[(2-Iodoacetyl)amino]ethyl]-5-methyl-2-oxo-4-imidazolidinehexanamide, DBIA, Desthiobiotin-IAA,Iodoacetamide-desthiobiotin
• Empirische Formel (Hill-System): C₁₄H₂₅IN₄O₃
• CAS-Nummer: 2924824-04-2
• Molekulargewicht: 424.28
• NACRES: NA.21
• Assay: ≥98% (HPLC)
• Form: powder

Eigenschaften

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C
• SMILES string: ICC(NCCNC(CCCCC[C@H]1NC(N[C@H]1C)=O)=O)=O

Beschreibung

Application

Desthiobiotin-iodoacetamide may be incorporated into the design of nanomaterials for applications in biosensing and imaging due to its chemical properties. It may also be used as a probe for cysteine-reactive affinity labeling in chemoproteomics, enabling the study of protein interactions and modifications.

Biochem/physiol Actions

The desthiobiotin-iodoacetamide (DBIA) probe represents a significant advancement in cysteine-reactive affinity labeling, surpassing alkyne-iodoacetamide in enrichment efficacy and mass spectrometry compatibility. With the ability to profile over 8,000 ligandable cysteine sites within 3 hours at 0.5-1 mM concentration, DBIA offers unparalleled proteome coverage and efficiency. Its unique feature of quantitative release from avidin under mild acidic conditions in organic solvents enhances its versatility in proteomic analyses. DBIA′s applicability to live cell cultures further expands its utility, positioning it as a powerful tool for investigating cysteine reactivity and protein dynamics in physiologically relevant contexts.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable