SML4297

H-Gly-Phe-AFC trifluoroacetate salt

≥98% (HPLC)

• Synonym(e): Gly-Phe-7-amino-4-trifluoromethylcoumarin trifluoroacetate salt, Gly-Phe-AFC; Glycylphenylalanyl-aminofluorocoumarin trifluoroacetate salt
• Empirische Formel (Hill-System): C₂₁H₁₈F₃N₃O₄ · xC₂HF₃O₂
• Molekulargewicht: 433.38 (free base basis)
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: powder
• Storage condition: desiccated

Eigenschaften

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• storage condition: desiccated
• color: white to beige
• solubility: H₂O: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Beschreibung

Biochem/physiol Actions

H-Gly-Phe-AFC (GF-AFC) is a cell-permeable dipeptidyl peptidase 1 (DPP1; cathepsin C; cathepsin J) substrate commonly used for cell viability accessment, where the fluorophore AFC is cleaved off by intracellular DPP1 activity in live cells. Unlike ATP and MTT assays, GF-AFC assay requires no lysis at the end of the assay, so cells can be used afterwards for other live cell-based assays. Cells are typically incubated with GF-AFC at a final concentration of 80 µM (from a 100 mM stock in DMSO) for 0.5-1 h at 37°C, and the AFC fluorescence intensity can be monitored by a fluorescence plate reader or by a fluorescence microscope (Ex 380-400 nm and Em 505 nm).

Disclaimer

Hygroscopic, store with desiccant

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable