SML4397

Azide-PEG3-Desthiobiotin

≥98% (HPLC)

• Synonym(e): (4R,5S)-Desthiobiotin-PEG3-Azide, Desthiobiotin-PEG3-Azide, Desthiobiotin-Azide, N3-DTB, DTB-PEG-N3, (4R,5S)-N-[2-[2-[2-(2-Azidoethoxy)ethoxy]ethoxy]ethyl]-5-methyl-2-oxo-4-imidazolidinehexanamide
• Empirische Formel (Hill-System): C₁₈H₃₄N₆O₅
• CAS-Nummer: 1426828-06-9
• Molekulargewicht: 414.50
• UNSPSC Code: 12352200
• Assay: ≥98% (HPLC)
• Form: (Crystal, liquid, powder, solid, semi-solid)

Eigenschaften

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: (Crystal, liquid, powder, solid, semi-solid)
• color: colorless to yellow
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C

Beschreibung

Biochem/physiol Actions

Desthiobiotin-PEG3-Azide is a trifunctional chemical probe designed for the chemoselective labeling and affinity enrichment of alkyne-modified biomolecules. Its strategic design combines a terminal azide that forms a stable triazole adduct with alkyne-tagged molecules via bio-orthogonal click chemistry—either catalyzed by Copper(I) (CuAAC) or proceeding catalyst-free with strained alkynes (SPAAC)—a hydrophilic PEG3 spacer to enhance solubility, and a desthiobiotin affinity tag. The probe′s pivotal advantage is the reversible, high-affinity binding of desthiobiotin to streptavidin (Kd ≈ 10⁻¹¹ M), which permits gentle, competitive elution that preserves the integrity of captured proteins and their interaction partners. Consequently, it serves as a central reagent in chemical proteomics for the affinity pull-down, enrichment, and subsequent mass spectrometry-based identification of alkyne-labeled biomolecules from complex cellular lysates, proving indispensable where the recovery of viable protein complexes is critical.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable