SRP4580

BMP-14 (GDF-5/CDMP-1) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): BMP-14/ CDMP-1, Cartilage derived morphogenetic protein-1, GDF-5, bone morphogenetic protein 14
• NACRES: NA.32
• UNSPSC Code: 12352202

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 0.5-4 μg ED₅₀
• mol wt: ~27 kDa
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: X80915
• UniProt accession no.: P43026
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... GDF5(8200)

Beschreibung

General description

The gene BMP-14 (bone morphogenetic protein 14) is mapped to human chromosome 20q11.22. It belongs to the bone morphogenetic protein family. Recombinant human BMP-14 is a 27kDa homodimeric protein consisting of two 120 amino acid polypeptide chains.

Biochem/physiol Actions

BMP-14 (bone morphogenetic protein 14) is involved in enhancing proliferations of osteoblasts, periosteum cells and connective tissue fibroblasts. It also promotes endochondral bone growth, proper development of limb skeletons and joints, and odontogenesis. It interacts with BMP type II receptor (BMPR-II) and might be involved in bone morphogenesis. Mutations in the BMP-14 gene are associated with brachydactyly type C, intervertebral disc degeneration, acromesomelic dysplasia Grebe type and osteoarthritis.

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 3.5.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute to a concentration of 0.1-1.0 mg/mL in water containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable