T4202

TRKA (440-end), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): DKFZp781l14186, MTC, NTRK1, TRK1, p140-TrKA
• CAS-Nummer: 152787-58-1
• NACRES: NA.32
• UNSPSC Code: 12352200
• eCl@ss: 32160702
• Specific activity: 19-25 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf9 cells

Eigenschaften

• biological source: human
• Quality Segment: 200
• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 19-25 nmol/min·mg
• mol wt: ~66 kDa
• technique(s): activity assay: suitable
• UniProt accession no.: P04629
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... NTRK1(4914)

Beschreibung

Application

TRKA (440-end), active, GST tagged human was used to study functional NGF signal transduction.

Biochem/physiol Actions

TRKA (440-end), active, GST tagged human is a 140 kD membrane-spanning protein tyrosine kinase that belongs to proto-oncogene family. TRKA facilitates the regulation of cell differentiation and survival via activating SHC1 and FRS2 which in turn activates a GRB2-Ras-MAPK cascade. It also plays a pivotal role in controlling NF-κ-B activation as well as transcription of genes involved in cell survival via PLCG1.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)