T7927

L-Tyrosin-Decarboxylase-Apoenzym aus Streptococcus faecalis

<0.005 unit/mg solid (without pyridoxal 5-phosphate), ≥0.05 unit/mg solid (with excess pyridoxal 5-phosphate)

• Synonym(e): L-(−)-Tyrosin-Apodecarboxylase, TAD
• CAS-Nummer: 9002-09-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-652-9
• MDL number: MFCD00131472
• EG-Nummer: 4.1.1.25 (BRENDA, IUBMB)
• eCl@ss: 32160703
• Specific activity: ≥0.05 unit/mg solid (with excess pyridoxal 5-phosphate), <0.005 unit/mg solid (without pyridoxal 5-phosphate)
• Biological source: Streptococcus faecalis

Eigenschaften

• biological source: Streptococcus faecalis
• Quality Segment: 200
• form: powder
• specific activity: ≥0.05 unit/mg solid (with excess pyridoxal 5-phosphate), <0.005 unit/mg solid (without pyridoxal 5-phosphate)
• storage temp.: −20°C

Beschreibung

Application

L-Tyrosine decarboxylase apoenzyme from Streptococcus faecalis has been used:

• in a study to purify and characterize tyrosine decarboxylase and aromatic-L-amino-acid decarboxylase
• in a study to investigate the stereospecificity of sodium borohydride reduction of tyrosine decarboxylase
• in protein synthesis studies

Biochem/physiol Actions

L-Tyrosine Decarboxylase Apoenzyme is an aromatic L-amino acid decarboxylase. The enzyme activity of tyrosine decarboxylase (TDC) is dependent on the cofactor pyridoxal 5-phosphate (PLP). It catalyzes the decarboxylation of dihydroxyphenylalanine.

Other Notes

Dried cells grown on vitamin B₆-deficient medium.

One unit will liberate 1.0 μmole of CO₂ from L-tyrosine per min at pH 5.5 at 37 °C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)