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  • Blockade of Oncogenic NOTCH1 with the SERCA Inhibitor CAD204520 in T Cell Acute Lymphoblastic Leukemia.

Blockade of Oncogenic NOTCH1 with the SERCA Inhibitor CAD204520 in T Cell Acute Lymphoblastic Leukemia.

Cell chemical biology (2020-05-11)
Matteo Marchesini, Andrea Gherli, Anna Montanaro, Laura Patrizi, Claudia Sorrentino, Luca Pagliaro, Chiara Rompietti, Samuel Kitara, Sabine Heit, Claus E Olesen, Jesper V Møller, Monia Savi, Leonardo Bocchi, Rocchina Vilella, Federica Rizzi, Marilena Baglione, Giorgia Rastelli, Caterina Loiacono, Roberta La Starza, Cristina Mecucci, Kimberly Stegmaier, Franco Aversa, Donatella Stilli, Anne-Marie Lund Winther, Paolo Sportoletti, Maike Bublitz, William Dalby-Brown, Giovanni Roti
ZUSAMMENFASSUNG

The identification of SERCA (sarco/endoplasmic reticulum calcium ATPase) as a target for modulating gain-of-function NOTCH1 mutations in Notch-dependent cancers has spurred the development of this compound class for cancer therapeutics. Despite the innate toxicity challenge associated with SERCA inhibition, we identified CAD204520, a small molecule with better drug-like properties and reduced off-target Ca2+ toxicity compared with the SERCA inhibitor thapsigargin. In this work, we describe the properties and complex structure of CAD204520 and show that CAD204520 preferentially targets mutated over wild-type NOTCH1 proteins in T cell acute lymphoblastic leukemia (T-ALL) and mantle cell lymphoma (MCL). Uniquely among SERCA inhibitors, CAD204520 suppresses NOTCH1-mutated leukemic cells in a T-ALL xenografted model without causing cardiac toxicity. This study supports the development of SERCA inhibitors for Notch-dependent cancers and extends their application to cases with isolated mutations in the PEST degradation domain of NOTCH1, such as MCL or chronic lymphocytic leukemia (CLL).

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Methanol, suitable for HPLC, ≥99.9%
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Kaliumchlorid, ACS reagent, 99.0-100.5%
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Natriumbicarbonat, ACS reagent, ≥99.7%
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N,N-Dimethylformamid, anhydrous, 99.8%
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Fetales Kälberserum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
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Fibronektin-Rinderplasma, solution, sterile-filtered, BioReagent, suitable for cell culture
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MOPS, ≥99.5% (titration)
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DAPI, for nucleic acid staining
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TWEEN® 80, viscous liquid
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L-Ascorbinsäure, BioXtra, ≥99.0%, crystalline
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Natriumcitrat-Dihydrat, ≥99%, FG
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Taurin, ≥99%
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