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  • Oral bioaccessibility of toxic and essential elements in raw and cooked commercial seafood species available in European markets.

Oral bioaccessibility of toxic and essential elements in raw and cooked commercial seafood species available in European markets.

Food chemistry (2018-06-24)
Ricardo N Alves, Ana L Maulvault, Vera L Barbosa, Margarita Fernandez-Tejedor, Alice Tediosi, Michiel Kotterman, Fredericus H M van den Heuvel, Johan Robbens, José O Fernandes, Rie Romme Rasmussen, Jens J Sloth, António Marques
ZUSAMMENFASSUNG

The oral bioaccessibility of several essential and toxic elements was investigated in raw and cooked commercially available seafood species from European markets. Bioaccessibility varied between seafood species and elements. Methylmercury bioaccessibility varied between 10 (octopus) and 60% (monkfish). Arsenic (>64%) was the toxic element showing the highest bioaccessibility. Concerning essential elements bioaccessibility in raw seafood, selenium (73%) and iodine (71%) revealed the highest percentages. The bioaccessibility of elements in steamed products increased or decreased according to species. For example, methylmercury bioaccessibility decreased significantly after steaming in all species, while zinc bioaccessibility increased in fish (tuna and plaice) but decreased in molluscs (mussel and octopus). Together with human exposure assessment and risk characterization, this study could contribute to the establishment of new maximum permissible concentrations for toxic elements in seafood by the European food safety authorities, as well as recommended intakes for essential elements.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Albumin aus Rinderserum, heat shock fraction, pH 7, ≥98%
Sigma-Aldrich
Calciumchlorid Dihydrat, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Harnstoff, ACS reagent, 99.0-100.5%
Sigma-Aldrich
Mucin aus Schweinemagen, Type II
Sigma-Aldrich
Harnsäure, ≥99%, crystalline
Sigma-Aldrich
Trypsin aus Schweinepankreas, Proteomics Grade, BioReagent, Dimethylated
Sigma-Aldrich
αα-Chymotrypsin aus Rinderpankreas, Type II, lyophilized powder, ≥40 units/mg protein
Sigma-Aldrich
Pepsin aus Schweinemagenschleimhaut, powder, ≥400 units/mg protein
Sigma-Aldrich
Lipase aus Schweinepankreas, Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Sigma-Aldrich
D-(+)-Glucosamin -hydrochlorid, ≥99% (HPLC), powder
Sigma-Aldrich
Bile extract porcine
Sigma-Aldrich
α-Amylase aus Aspergillus oryzae, powder, ~1.5 U/mg (~0.2 U acc. to Willstätter)