Lucilia sericata chymotrypsin disrupts protein adhesin-mediated staphylococcal biofilm formation.

Lucilia sericata chymotrypsin disrupts protein adhesin-mediated staphylococcal biofilm formation.

Applied and environmental microbiology (2012-12-12)

Llinos G Harris, Yamni Nigam, James Sawyer, Dietrich Mack, David I Pritchard

PMID23220967

ZUSAMMENFASSUNG

Staphylococcus aureus and Staphylococcus epidermidis biofilms cause chronic infections due to their ability to form biofilms. The excretions/secretions of Lucilia sericata larvae (maggots) have effective activity for debridement and disruption of bacterial biofilms. In this paper, we demonstrate how chymotrypsin derived from maggot excretions/secretions disrupts protein-dependent bacterial biofilm formation mechanisms.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

C4129

Sigma-Aldrich

αα-Chymotrypsin aus Rinderpankreas, Type II, lyophilized powder, ≥40 units/mg protein

C3142

Sigma-Aldrich

αα-Chymotrypsin, (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

C7762

Sigma-Aldrich

αα-Chymotrypsin aus Rinderpankreas, Type I-S, essentially salt-free, lyophilized powder

CHY5S

Sigma-Aldrich

αα-Chymotrypsin aus Rinderpankreas, ≥40 units/mg protein, vial of 5 mg

C6423

Sigma-Aldrich

αα-Chymotrypsin aus Rinderpankreas, suitable for protein sequencing, salt-free, lyophilized powder

C8946

Sigma-Aldrich

α-Chymotrypsin from human pancreas, lyophilized powder

C9134

Sigma-Aldrich

α-Chymotrypsin−Agarose from bovine pancreas, lyophilized powder, 2,000-3,500 units/g agarose (One ml gel will yield 65-120 units)