- Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.
Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.
Journal of agricultural and food chemistry (2004-08-19)
Dawn M Chapman, John H Thorngate, Mark A Matthews, Jean-Xavier Guinard, Susan E Ebeler
PMID15315381
ZUSAMMENFASSUNG
A rapid and automated solid phase microextraction (SPME) stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for 2-methoxy-3-isobutylpyrazine (MIBP) quantification in red wine was developed. Wines with 30% (w/v) NaCl and 2-methoxy-(2)H(3)-3-isobutylpyrazine internal standard were sampled with a 2 cm divinylbenzene/carboxen/poly(dimethylsiloxane) SPME fiber for 30 min at 40 degrees C and analyzed by GC-MS. The method was used to measure MIBP concentrations in Cabernet Sauvignon wines that were produced from six winter pruning treatments over two vintages. MIBP concentrations were significantly negatively correlated with buds per vine. In addition, the MIBP concentration was directly related to sensory vegetal intensity ratings obtained by descriptive analysis.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Supelco
SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub
Supelco
SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 24 ga, Autosampler, pk of 3, notched gray hub
Supelco
SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub
Supelco
SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub
Supelco
SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub
Supelco
ORBO™ Carboxen-564 Röhrchen, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)
Supelco
ORBO™ 78 HBr auf Carboxen® 564 speziell gereinigt (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, black hub
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, black hub
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, light blue hub
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub
Supelco
ORBO™-93 Sorbensröhrchen, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, black hub
Supelco
Carboxen®-1006 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.32 mm, average thickness 15 μm
Supelco
SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, black hub
Supelco
Tenax® TA / Carboxen® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok® End-Fittings), pkg of 10 ea
Supelco
SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, Metal Alloy (2cm), needle size 23 ga, Autosampler, pk of 1, plain gray hub
Supelco
Carboxen® 572 for Tobacco Smoke, Adsorbent Bed Wt: 300 mg, cartridge size 3 mL, pkg of 50 ea
Supelco
SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, Metal Alloy (1cm), needle size 23 ga, Autosampler, pk of, plain gray hub