Effect of gold nanoparticles on photodynamic efficiency of 5-aminolevolenic acid photosensitiser in epidermal carcinoma cell line: an in vitro study.

Effect of gold nanoparticles on photodynamic efficiency of 5-aminolevolenic acid photosensitiser in epidermal carcinoma cell line: an in vitro study.

IET nanobiotechnology (2013-11-12)

Seyed Mohammad Amini, Sharmin Kharrazi, Mahnaz Hadizadeh, Mohsen Fateh, Reza Saber

PMID24206772

ZUSAMMENFASSUNG

In the recent years, enhanced functionality of treatment systems based on nanostructures has attracted a lot of interest. Photodynamic therapy (PDT) is one such treatment method. Here, the authors report the results of the investigations on synthesis and characterisation of gold nanoparticles (GNPs) and their application in PDT along with 5-aminolevolenic acid (5-ALA) (as photosensitiser) with no conjugation. Three sizes of GNPs were synthesised and their cytotoxicity was investigated by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on epidermal carcinoma cell line. The results showed that the PDT efficiency of ALA increased in presence of GNPs. This effect was more considerable for 4 nm particles.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

M2128

Sigma-Aldrich

Thiazolylblau-Tetrazoliumbromid, 98%

M5655

Sigma-Aldrich

Thiazolylblau-Tetrazoliumbromid, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

265837

Sigma-Aldrich

Gold, rod, diam. 3.0 mm, 99.99% trace metals basis

265772

Sigma-Aldrich

Gold, powder, <45 μm, 99.99% trace metals basis

636347

Sigma-Aldrich

Gold, nanopowder, <100 nm particle size, 99.9% trace metals basis

326585

Sigma-Aldrich

Gold, powder, <10 μm, ≥99.9% trace metals basis

643203

Sigma-Aldrich

Goldbeschichteter Glasobjektträger, layer thickness 100 Å, 99.999% (Au)

326534

Sigma-Aldrich

Gold, wire, diam. 0.25 mm, ≥99.9% trace metals basis

326542

Sigma-Aldrich

Gold, beads, 1-6 mm, 99.999% trace metals basis

265810

Sigma-Aldrich

Gold, foil, thickness 0.1 mm, 99.99% trace metals basis

643246

Sigma-Aldrich

Goldbeschichteter Glasobjektträger, layer thickness 1000 Å, 99.999% (Au)

349275

Sigma-Aldrich

Gold, foil, thickness 0.05 mm, 99.99% trace metals basis

349283

Sigma-Aldrich

Gold, wire, diam. 0.1 mm, 99.99% trace metals basis

265829

Sigma-Aldrich

Gold, foil, thickness 0.5 mm, 99.99% trace metals basis

310980

Sigma-Aldrich

Gold, wire, diam. 0.5 mm, 99.99% trace metals basis

265799

Sigma-Aldrich

Gold, wire, diam. 1.0 mm, 99.99% trace metals basis

326496

Sigma-Aldrich

Gold, foil, thickness 0.127 mm, 99.99% trace metals basis

349240

Sigma-Aldrich

Gold, foil, thickness 0.25 mm, ≥99.9% trace metals basis

373184

Sigma-Aldrich

Gold, evaporation slug, diam. × L 0.3 cm × 0.6 cm, 99.99% trace metals basis

255734

Sigma-Aldrich

Gold, powder, <850 μm, ≥99.99% trace metals basis

349305

Sigma-Aldrich

Gold, wire, diam. 1.0 mm, 99.997% trace metals basis

265780

Sigma-Aldrich

Gold, wire, diam. 0.25 mm, 99.99% trace metals basis

268461

Sigma-Aldrich

Gold, foil, thickness 0.025 mm, 99.99% trace metals basis

643254

Sigma-Aldrich

Goldbeschichtete Glasabdeckfolie, layer thickness 100 Å, 99.999% (Au), L × W × thickness 22 mm × 22 mm × 130-170 μm, square

349313

Sigma-Aldrich

Gold, wire, diam. 0.5 mm, 99.999% trace metals basis

373168

Sigma-Aldrich

Gold, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis

643289

Sigma-Aldrich

Goldbeschichtete Glasabdeckfolie, 99.999% (Au), diam. × thickness 15 mm × 130-170 μm, layer thickness 100 Å

326526

Sigma-Aldrich

Gold, wire, diam. 0.127 mm, 99.99% trace metals basis