Direkt zum Inhalt
Merck
  • Differential contribution of ROS to resveratrol-induced cell death and loss of self-renewal capacity of ovarian cancer stem cells.

Differential contribution of ROS to resveratrol-induced cell death and loss of self-renewal capacity of ovarian cancer stem cells.

Anticancer research (2015-01-01)
Manabu Seino, Masashi Okada, Keita Shibuya, Shizuka Seino, Shuhei Suzuki, Hiroyuki Takeda, Tsuyoshi Ohta, Hirohisa Kurachi, Chifumi Kitanaka
ZUSAMMENFASSUNG

Cancer stem cells (CSCs) are considered to contribute to the poor prognosis of ovarian cancer as a major cause of fatal recurrence. Identification of effective measures to eliminate ovarian CSCs through induction of cell death and/or loss of self-renewal capacity would, therefore, be key to successful management of ovarian cancer. The effects of resveratrol on the viability and self-renewal capacity of CSCs derived from A2780 human ovarian cancer cells were examined. The involvement of reactive oxygen species (ROS) was also investigated. At a non-toxic to normal human fibroblasts concentration, resveratrol effectively killed ovarian CSCs independently of ROS, while ROS-dependently impaired the self-renewal capacity of ovarian CSCs that survived resveratrol treatment. Our findings not only shed light on a novel mechanism of action for resveratrol but also suggest that resveratrol, or its analogs, may be useful for CSC-directed therapy against ovarian cancer.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Dimethylsulfoxid, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Dimethylsulfoxid, ACS reagent, ≥99.9%
Sigma-Aldrich
Dimethylsulfoxid, Molecular Biology
Sigma-Aldrich
Dimethylsulfoxid, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
Sigma-Aldrich
Wasserstoffperoxid -Lösung, contains inhibitor, 30 wt. % in H2O, ACS reagent
Sigma-Aldrich
Dimethylsulfoxid, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Dimethylsulfoxid, anhydrous, ≥99.9%
Sigma-Aldrich
Wasserstoffperoxid -Lösung, 30 % (w/w) in H2O, contains stabilizer
Sigma-Aldrich
Natriumdodecylsulfat, BioReagent, Molecular Biology, ≥98.5% (GC)
Sigma-Aldrich
Dimethylsulfoxid, ≥99.5% (GC), suitable for plant cell culture
Sigma-Aldrich
N-Acetyl-L-Cystein, BioReagent, suitable for cell culture
Sigma-Aldrich
Natriumfluorid, ACS reagent, ≥99%
Sigma-Aldrich
Natriumdodecylsulfat, ≥99.0% (GC), dust-free pellets
Sigma-Aldrich
Natriumchlorid, Molecular Biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
Natriumchlorid -Lösung, 5 M in H2O, BioReagent, Molecular Biology
Sigma-Aldrich
Natriumchlorid, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
Natriumchlorid, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Natriumchlorid -Lösung, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
Natriumdodecylsulfat, ACS reagent, ≥99.0%
Sigma-Aldrich
Natriumdodecylsulfat -Lösung, BioUltra, Molecular Biology, 10% in H2O
Sigma-Aldrich
N-Acetyl-L-Cystein, Sigma Grade, ≥99% (TLC), powder
Sigma-Aldrich
Wasserstoffperoxid -Lösung, 50 wt. % in H2O, stabilized
Sigma-Aldrich
Deoxycholsäure Natriumsalz, BioXtra, ≥98.0% (dry matter, NT)
Sigma-Aldrich
Deoxycholsäure Natriumsalz, ≥97% (titration)
Sigma-Aldrich
Natriumdodecylsulfat, ReagentPlus®, ≥98.5% (GC)
Sigma-Aldrich
Ethylendiamintetraessigsäure, 99.4-100.6%, powder
Sigma-Aldrich
Resveratrol, ≥99% (HPLC)
Sigma-Aldrich
Dimethylsulfoxid, BioUltra, Molecular Biology, ≥99.5% (GC)
Sigma-Aldrich
Ethylendiamintetraessigsäure, anhydrous, crystalline, BioReagent, suitable for cell culture