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D8418

Dimethylsulfoxid

Name: Dimethylsulfoxid
Brand: SIGMA

Molecular Biology

Synonym(e):

DMSO für die Molekularbiologie, Methylsulfinylmethan, Methylsulfoxid, DMSO

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Größe/SKU	Verfügbarkeit	Preis

Über diesen Artikel

Lineare Formel:

(CH₃)₂SO

CAS-Nummer:

67-68-5

Molekulargewicht:

78.13

UNSPSC Code:

12191502

NACRES:

NA.52

PubChem Substance ID:

24894214

EC Number:

200-664-3

Beilstein/REAXYS Number:

506008

MDL number:

MFCD00002089

Assay:

≥99.9%

Grade:

Molecular Biology

Technique(s):

DNA sequencing: suitable, PCR: suitable, transfection: suitable

Bp:

189 °C (lit.)

Vapor pressure:

0.42 mmHg ( 20 °C)

Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Technischer Dienst

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Eigenschaften

grade

Molecular Biology

Quality Segment

200

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

product line

BioReagent

assay

≥99.9%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

technique(s)

DNA sequencing: suitable, PCR: suitable, transfection: suitable

impurities

≤0.001 meq/g Titratable acid, ≤0.1% water (Karl Fischer)

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H₂O: miscible (completely)

density

1.10 g/mL (lit.)

absorption

passes test

suitability

suitable for molecular biology

foreign activity

DNase and RNase, none detected

storage temp.

room temp

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Beschreibung

General description

Eigenschaften und Vorteile:

Geeignet für molekulare Anwendungen
Nuklease-freies DMSO
DNasefrei, RNasefrei, Proteasefrei, Phosphatasefrei
DMSO für die Molekularbiologie

Dimethylsulfoxid (DMSO) ist ein hochpolares organisches Reagenz, das über außergewöhnliche Lösungsmitteleigenschaften für organische und anorganische Chemikalien verfügt.
Dieses Produkt ist als molekularbiologisches Reagenz klassifiziert und ist für molekularbiologische Anwendungen geeignet. Es wurde auf das Vorhandensein von Nukleasen und Proteasen hin analysiert.

Application

DMSO (Dimethylsulfoxid) ist ein hochpolares, aprotisches organisches Lösungsmittel mit vielen Einsatzmöglichkeiten in der organischen Chemie und Molekularbiologie. DMSO wird routinemäßig verwendet in

Polymerase-Kettenreaktion (PCR)
Amplifikation von cDNA-Bibliotheken
DNA-Sequenzierung
Säulen-Ladepuffer für die Poly(A)+ RNA-Selektion
Puffer für die Transformation kompetenter E. coli
Transfektionsprotokolle

Dimethylsulfoxid wird verwendet:

als Lösungsmittel für Oligonukleotide beim Array-Spotting[1]
als Lösungsmittel bei der miRNA-Microarray-Analyse und der quantitativen Echtzeit-PCR (qRT-PCR)
für die Zelllyse während des MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium-Bromid)-Assays
für die Herstellung der Stammlösung während der zytofluorimetrischen Bewertung des MMP (mitochondriales Membranpotential)
bei der Lagerung menschlicher und tierischer Zelllinien und Bakteriophage λ als Kryoprotektor.

Zur Herstellung einer steril gefilterten DMSO-Lösung wird die Verwendung einer PTFE- oder Nylonmembran empfohlen. Membranen aus Celluloseacetat werden nicht empfohlen.

Features and Benefits

Dieses Produkt ist DNasefrei, RNasefrei, Phosphatasefrei und Proteasefrei.

Other Notes

Gewinner des CiteAb Award 2024 in der Kategorie „Supplier Succeeding in Parkinson′s Research“ (Erfolgreiche Anbieter in der Parkinson-Forschung)

Disclaimer

Unterkühlt leicht und schmilzt langsam bei Raumtemperatur. Das verfestigte Produkt kann durch Erwärmen auf Raumtemperatur wieder verflüssigt werden, ohne dabei geschädigt zu werden.

Questions

1–10 of 19 Questions

2 years ago

Is this product anhydrous?

1 answer
1. Technical Support
  
  a year ago
  
  This product is not anhydrous. The maximum allowable water content is < 0.1%. Please see the link below to review an anhydrous product option.
  276855
  https://www.sigmaaldrich.com/product/sial/276855
  
  Helpful?
3 years ago

May I know the concentration (mg/ml) for DMSO (cat no. D8418)?

1 answer
1. Technical Support
  
  3 years ago
  
  This product is supplied as a neat or pure liquid with a density of 1.10 g/mL and a molecular weight of 78.13 g/mol. The minimum purity is 99.9%, thus the molarity will vary slightly from lot to lot. The molarity is 14.1 M.
  
  Helpful?
3 years ago

Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?

1 answer
1. Technical Support
  
  3 years ago
  
  Yes, Dimethyl sulfoxide (DMSO) is generally considered to be compatible with Polyethylene terephthalate (PET) material. The following information can be found on the Product Data Sheet:
  -Compatible: LDPE, HDPE, polypropylene, PPCO (polypropylene copolymer), polymethylpentene, nylon, teflon FEP
  -Moderately compatible: polystyrene, ECTFE/ETFE
  -Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265/d2650pis.pdf
  
  Helpful?
3 years ago

Is this product sterile?

1 answer
1. Technical Support
  
  3 years ago
  
  This product is not sterile. To prepare a sterile filtered DMSO solution, it is recommended to use a Teflon, polypropylene, or nylon membrane. See products D2650 or D2438 for sterile DMSO product options. Please review these products at the links provided below:
  https://www.sigmaaldrich.com/product/sigma/d2650
  https://www.sigmaaldrich.com/product/sigma/d2438
  
  Helpful?
Anonymous

4 years ago

What can I use to solubilize Product D8418, Dimethyl sulfoxide?

1 answer
1. Technical Support
  
  4 years ago
  
  This product is miscible in water (1 mL DMSO + 1 mL H2O), yielding a clear, colorless solution. DMSO is a very hygroscopic liquid and should protected from exposure to moisture. DMSO is also miscible with ethanol, acetone, ether, benzene, and chloroform.
  
  Helpful?
Anonymous

4 years ago

I received Product D8418, Dimethyl sulfoxide, as a frozen solid. But isn't it supposed to be in the liquid state?

1 answer
1. Technical Support
  
  4 years ago
  
  DMSO super cools easily and remelts slowly at room temperature. The product may arrive as a solid instead of a liquid. The solidified product can be reliquified by warming to room temperature without detriment to the product.
  
  Helpful?
Anonymous

4 years ago

Dimethyl sulfoxide (DMSO) should be a liquid. Why did it arrive as a solid?

1 answer
1. Technical Support
  
  4 years ago
  
  DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
  
  Helpful?
Anonymous

4 years ago

What is the molarity of DMSO?

1 answer
1. Technical Support
  
  4 years ago
  
  DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
  
  Helpful?
Anonymous

4 years ago

Why is Product D8418, Dimethyl sulfoxide, considered as an exceptional organic solvent?

1 answer
1. Technical Support
  
  4 years ago
  
  Dimethyl sulfoxide (DMSO) is a highly polar organic reagent that has exceptional solvent properties for organic and inorganic chemicals.
  
  Helpful?
Anonymous

4 years ago

Where does DMSO come from?

1 answer
1. Technical Support
  
  4 years ago
  
  DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
  
  Helpful?

1–10 of 19 Questions

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Overall, average rating value is 4.5 of 5.

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2 Ratings-only reviews

Dieser Artikel	PHR1309	D9170	94563
Sigma-Aldrich D8418 Dimethylsulfoxid	Supelco PHR1309 Dimethyl Sulfoxide	Sigma-Aldrich D9170 Dimethylsulfoxid	Supelco 94563 Dimethylsulfoxid
description Molecular Biology	description Pharmaceutical Secondary Standard; Certified Reference Material	description PCR Reagent	description analytical standard
technique(s) transfection: suitable, DNA sequencing: suitable, PCR: suitable	technique(s) gas chromatography (GC): suitable, HPLC: suitable	technique(s) PCR: suitable	technique(s) gas chromatography (GC): suitable, HPLC: suitable
assay ≥99.9%	assay -	assay ≥99.5%	assay ≥99.95% (GC)
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 100
grade Molecular Biology	grade pharmaceutical secondary standard, certified reference material	grade -	grade analytical standard
solubility H₂O: miscible (completely)	solubility -	solubility H₂O: miscible (completely)	solubility H₂O: miscible (completely)
form liquid	form -	form liquid	form -

Wichtige Dokumente

Fortfahren mit

Dimethylsulfoxid

Molecular Biology

Größe auswählen

Über diesen Artikel

grade

Quality Segment

vapor density

vapor pressure

product line

assay

form

autoignition temp.

shelf life

expl. lim.

technique(s)

impurities

color

refractive index

bp

mp

solubility

density

absorption

suitability

foreign activity

storage temp.

SMILES string

InChI

InChI key

General description

Application

Features and Benefits

Other Notes

Disclaimer

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Questions

Angelo

Is this product anhydrous?

Anonymous

May I know the concentration (mg/ml) for DMSO (cat no. D8418)?

Anonymous

Is Product D8418, Dimethyl sulfoxide, also compatible with PET material?

Alesia66

Is this product sterile?

Reviews

Rating Snapshot

Average Customer Ratings

Active Filters

2 Ratings-only reviews