Steady-state cross-correlations for live two-colour super-resolution localization data sets.

Steady-state cross-correlations for live two-colour super-resolution localization data sets.

Nature communications (2015-06-13)

Matthew B Stone, Sarah L Veatch

ZUSAMMENFASSUNG

Cross-correlation of super-resolution images gathered from point localizations allows for robust quantification of protein co-distributions in chemically fixed cells. Here this is extended to dynamic systems through an analysis that quantifies the steady-state cross-correlation between spectrally distinguishable probes. This methodology is used to quantify the co-distribution of several mobile membrane proteins in both vesicles and live cells, including Lyn kinase and the B-cell receptor during antigen stimulation.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

H3375

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HEPES, ≥99.5% (titration)

H4034

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HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

M1028

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F8775

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