An indirect assay for volatile compound production in yeast strains.

An indirect assay for volatile compound production in yeast strains.

Scientific reports (2014-01-16)

Davide Ravasio, Andrea Walther, Kajetan Trost, Urska Vrhovsek, Jürgen Wendland

ZUSAMMENFASSUNG

Traditional flavor analysis relies on gas chromatography coupled to mass spectrometry (GC-MS) methods. Here we describe an indirect method coupling volatile compound formation to an ARO9-promoter-LacZ reporter gene. The resulting β-galactosidase activity correlated well with headspace solid phase micro extraction (HS/SPME) GC-MS data, particularly with respect to the formation of rose flavor. This tool enables large-scale screening of yeast strains and their progeny to identify the most flavor active strains.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

57298-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub

57348-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 24 ga, Autosampler, pk of 3, notched gray hub

57328-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

57299-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub

57341-U

Supelco

SPME-Faseranordnung Polydimethylsiloxan (PDMS), 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, red hub

57329-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub

57300-U

Supelco

SPME-Faseranordnung Polydimethylsiloxan (PDMS), 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, red hub

57318

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, black hub

57301

Supelco

SPME-Faseranordnung Polydimethylsiloxan (PDMS), 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, red hub

57310-U

Supelco

SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, blue hub

57319

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, black hub

57295-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub

57293-U

Supelco

SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), 65 μm PDMS/DVB, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, pink hub

57311

Supelco

SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, blue hub

57326-U

Supelco

SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, pink hub

57334-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, light blue hub

57305

Supelco

SPME-Faseranordnung Polyacrylat (PA), 85 μm Polyacrylate, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, white hub

57359-U

Supelco

Tragbarer SPME-Field-Sampler, coating PDMS/DVB

57327-U

Supelco

SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, pink hub

57294-U

Supelco

SPME-Faseranordnung Polyacrylat (PA), 85 μm Polyacrylate, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, white hub

57335-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

57346-U

Supelco

SPME-Faseranordnung Polydimethylsiloxan/Divinylbenzen (PDMS/DVB), 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, blue hub

504823

Supelco

Tragbarer SPME-Field-Sampler, coating PDMS

57550-U

Supelco

SPME StableFlex^™ Methodenentwicklungs-Kit, Assortment, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 4

57343-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, black hub

57342-U

Supelco

SPME-Faseranordnung Polydimethylsiloxan (PDMS), 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, red hub

57284-U

Supelco

SPME StableFlex^™ Methodenentwicklungs-Kit, Assortment, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 4

504831

Supelco

Tragbarer SPME-Field-Sampler, coating CAR/PDMS

57324-U

Supelco

SPME-Fasersortiment-Kit 4, Assortment #4, Fused Silica (1 cm), size 24 ga, Manual Holder, pk of 3

57354-U

Supelco

SPME-Faseranordnung, Carbowax-Polyethylenglycol (PEG)-Beschichtung, 60 μm PEG, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 3, purple hub