Solid-phase microextraction-gas chromatographic-mass spectrometric method for the determination of the fungicides cyprodinil and fludioxonil in white wines.

Solid-phase microextraction-gas chromatographic-mass spectrometric method for the determination of the fungicides cyprodinil and fludioxonil in white wines.

Journal of chromatography. A (2002-02-02)

Otero R Rial, Ruiz C Yagüe, Grande B Cancho, Gándara J Simal

ZUSAMMENFASSUNG

A solid-phase microextraction (SPME) followed by a gas chromatographic-mass spectrometric (GC-MS) determination has been developed and validated for the determination of cyprodinil and fludioxonil in white wine samples. Extraction parameters such as the selection of SPME coating, the effect of the temperature, the effect of the headspace volume and the salt addition were studied and optimized, together with GC-MS analytical conditions. The divinylbenzene-Carboxen-polydimethylsiloxane (DVB-CAR-PDMS) fiber was the most appropriate for the determination of the two pesticides in wine. The quality parameters of the proposed method demonstrated a good precision (RSD about 5%), with detection limits of 0.1 and 0.2 microg/l for cyprodinil and fludioxonil, respectively. Fifteen commercial white wine samples produced in Rías Baixas area in Galicia (N.W. Spain) were analyzed with the SPME-GC-MS procedure. Some of the commercial wines (75%) presented the two pesticides in concentrations ranging from 0.9 to 28.6 microg/l. In conclusion, SPME-GC-MS has a great potential for fungicide determination in wines.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

57298-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub

57348-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 24 ga, Autosampler, pk of 3, notched gray hub

57328-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

57299-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub

57329-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub

20358

Supelco

ORBO^™ Carboxen-564 Röhrchen, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

20355

Supelco

ORBO^™ 78 HBr auf Carboxen^® 564 speziell gereinigt (20/45), 400/200 mg, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

25467

Supelco

Carboxen^®-1010 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

10478-U

Supelco

Carboxen^® Adsorptionsmittel, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

57318

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, black hub

25461

Supelco

Carboxen^®-1006 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57319

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, black hub

57295-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub

24246

Supelco

Carboxen^®-1010 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

57334-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, light blue hub

57335-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

23446-U

Supelco

ORBO^™-93 Sorbensröhrchen, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea

57343-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, black hub

24241-U

Supelco

Carboxen^®-1006 PLOT Kapillar-GC-Säule, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

28718-U

Supelco

Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

57344-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, black hub

10471

Supelco

Carboxen^® Adsorptionsmittel, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

57914-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, Metal Alloy (2cm), needle size 23 ga, Autosampler, pk of 1, plain gray hub

54294-U

Supelco

Carboxen^® 572 for Tobacco Smoke, Adsorbent Bed Wt: 300 mg, cartridge size 3 mL, pkg of 50 ea

57912-U

Supelco

SPME-Faseranordnung, 50/30µm DVB/CAR/PDMS, Metal Alloy (1cm), needle size 23 ga, Autosampler, pk of, plain gray hub

10269

Supelco

Carboxen^® Adsorptionsmittel, matrix Carboxen^® 569, 20-45 mesh, bottle of 10 g

54041-U

Supelco

Carboxen^® 1000 Reversibles SPE-Röhrchen, 200 mg/mL, pk 30

10477-U

Supelco

Carboxen^® Adsorptionsmittel, matrix Carboxen^® 1000, 40-60 mesh, bottle of 50 g

11072-U

Supelco

Carboxen^® Adsorptionsmittel, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

57906-U

Supelco

SPME-Faseranordnung Carboxen/Polydimethylsiloxan (CAR/PDMS), 85 μm CAR/PDMS, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 1, light blue hub