H3662

HEPES sodium salt solution

BioReagent, 1M, suitable for cell culture

• Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
• CAS Number: 75277-39-3
• Molecular Weight: 260.29
• MDL number: MFCD00036463
• UNSPSC Code: 12161700
• PubChem Substance ID: 24895590
• NACRES: NA.25

Properties

• Quality Level: 200
• sterility: sterile-filtered (0.2 μm filtered)
• product line: BioReagent
• form: solution
• concentration: 0.90-1.10 M
• technique(s): cell culture | mammalian: suitable
• impurities: Bioburden, tested; DNase, RNase. Protease, Nickase, free; endotoxin, tested; <5 ppm Heavy metals (as Pb)
• cation traces: Fe: <5 ppm
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M

Description

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966.[1] It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts.[2] It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.[3]

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves