H7006

HEPES sodium salt

≥99.0% (titration)

• Synonym(s): Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
• Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
• CAS Number: 75277-39-3
• Molecular Weight: 260.29
• NACRES: NA.25
• PubChem Substance ID: 24895780
• UNSPSC Code: 12161700
• EC Number: 278-169-7
• MDL number: MFCD00036463

Properties

• Product Name: HEPES sodium salt, ≥99.0% (titration)
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• assay: ≥99.0% (titration)
• form: powder
• storage condition: dry at room temperature
• technique(s): HPLC: suitable
• color: white
• pH: 10-12 (1 M in water)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: water: 0.1 M, clear, colorless
• suitability: suitable for mass spectrometry
• application(s): diagnostic assay manufacturing; general analytical (; ); life science and biopharma; metabolomics
• storage temp.: room temp
• Quality Level: 400

Description

Application

HEPES buffer finds application in:

• Most studies with metal ions
• Substitute for Tris and phosphate in studies with metal ions
• Grinding buffer in plant studies
• Running buffer in gel electrophoresis
• Buffer in electroporation studies
• Bradford or bicinchoninic acid (BCA) assays
• Extraction buffer to prevent damage of proteins in red blood cells

It has been used:

• as a buffer component in the preparation of a low sucrose buffer[1]
• as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions[2]

Features and Benefits

• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
• Highly soluble in water
• Negligible metal ion binding
• Less toxic to cells than other buffers such as Tris and phosphate
• Stable in a wide pH range

General description

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

Other Notes

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Packaging

The DRYPOUR^®packaging system for high-quality hygroscopic salts DRYPOUR^® packaging:

• The current DRYPOUR^® packaging configuration is in a 50L nestable drum
• 25KG-DP are the standard prepack offering
• Smaller volumes can be put in the same drum for variable configurations

The benefits of DRYPOUR^®:

• Improves operational efficiency by reducing material preparation time and increasing operator safety
• Minimizes caking of hygroscopic salts
• Offers triple protection: against moisture inside and outside, and against contamination

How does the DRYPOUR^®packaging system Improve your operational efficiency?

• This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
• PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek^® liner
• Desiccant bags are firmly integrated into the polyethylene liner
• Triple protection delivers two invaluable results:

1. Dramatically reduced caking
2. No contamination risk from the desiccant

Physical form

Lyophilized, spray dried, or crystallized material.

Legal Information

DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany

Tyvek is a registered trademark of E. I. du Pont de Nemours and Company

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)