RDD035

HEPES sodium salt

≥99.5% (titration), free-flowing, Redi-Dri^™

• Synonym(s): HEPES Sodium, HEPES hemisodium salt, Sodium HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
• Empirical Formula (Hill Notation): C₈H₁₇N₂NaO₄S
• CAS Number: 75277-39-3
• Molecular Weight: 260.29
• UNSPSC Code: 12161700
• NACRES: NA.25
• MDL number: MFCD00036463

Properties

• Product Name: HEPES sodium salt, ≥99.5% (titration), free-flowing, Redi-Dri^™
• InChI: 1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
• SMILES string: [Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
• InChI key: RDZTWEVXRGYCFV-UHFFFAOYSA-M
• product line: Redi-Dri^™
• assay: ≥99.5% (titration)
• quality: free-flowing
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• application(s): diagnostic assay manufacturing
• Quality Level: 100

Description

Application

HEPES sodium salt can be used:

• as a protein precipitation buffer
• in cell culture media
• as a binding buffer

Features and Benefits

• Redi-Dri^™ eliminates clumping for hygroscopic chemicals
• powder remains free-flowing and easy to use
• Safer to handle and weigh out for standard use in lab
• Eliminates clumps, maintains product moisture, quality, and characteristics

General description

HEPES sodium salt is essential to prepare HEPES buffer that is commonly used for cell culture and protein applications. It is useful for maintaining homeostasis when working with cells outside of the incubator by balancing CO₂. HEPES buffering capacity exceeds most buffers, with a pKa of 7.5, making it optimal for maintaining pH in biological systems. In solution, it is non-toxic, zwitterion with a physiological buffering range of pH 6.8 - 8.2. It has a low metal binding capacity and is safe to use in research for metal-binding proteins.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Physical form

Lyophilized, spray dried, or crystallized material.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable