HSTCMAG-28SK

MILLIPLEX^® Human High Sensitivity T Cell Panel

Configurable 96-well Human High Sensitivity T Cell 21-Plex Panel

• Synonym(s): Human high-sensitivity cytokine multiplex kit, Luminex^® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel
• UNSPSC Code: 12161503
• NACRES: NA.47
• eCl@ss: 32161000

Properties

• species reactivity: human
• Quality Level: 200
• packaging: pkg of 1 ea
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 98-107%, sensitivity: 0.11-8.17 pg/mL; (Overnight Incubation minDC), standard curve range: 0.18-750 pg/mL; (IL-6), standard curve range: 0.24-1,000 pg/mL; (IL-13), standard curve range: 0.24-1,000 pg/mL; (IL-21), standard curve range: 0.31-1,250 pg/mL; (IL-8), standard curve range: 0.31-1,250 pg/mL; (MIP-1α), standard curve range: 0.37-1,500 pg/mL; (IL-7), standard curve range: 0.45-1,750 pg/mL; (TNFα), standard curve range: 0.49-2,000 pg/mL; (IL-1β), standard curve range: 0.49-2,000 pg/mL; (IL-12 (p70)), standard curve range: 0.49-2,000 pg/mL; (IL-2), standard curve range: 0.49-2,000 pg/mL; (IL-5), standard curve range: 0.61-2,500 pg/mL; (IFNγ), standard curve range: 0.61-2,500 pg/mL; (MIP-3α), standard curve range: 0.73-3,000 pg/mL; (IL-17A), standard curve range: 0.92-3,750 pg/mL; (MIP-1β), standard curve range: 1.22-5,000 pg/mL; (GM-CSF), standard curve range: 1.46-6,000 pg/mL; (IL-10), standard curve range: 1.46-6,000 pg/mL; (ITAC), standard curve range: 1.83-7,500 pg/mL; (IL-4), standard curve range: 18.3-75,000 pg/mL; (Fractalkine), standard curve range: 7.93-32,500 pg/mL; (IL-23), inter-assay cv: <15%; intra-assay cv: <10%
• technique(s): multiplexing: suitable
• input: serum; cell culture supernatant
• detection method: fluorometric (Luminex^® xMAP^® technology)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. Low levels of inflammation are involved in many sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

Application

MILLIPLEX^® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze up to 21 analytes in human serum, plasma, and cell culture supernatants.

Analytes included: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, I-TAC, MIP-1α, MIP-1β, MIP-3α, TNF-α

Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.

Features and Benefits

• High-Sensitivity Detection: Enables the detection of low-abundance T cell cytokines, providing critical insights into subtle immune responses and enhancing understanding of disease mechanisms.
• Simultaneous Quantification of Multiple Analytes: Capable of measuring a variety of T cell-related cytokines and chemokines in a single assay run, including IL-1β, IL-2, IL-6, IL-10, TNFα, and others, saving time and resources.
• Versatile Sample Compatibility: Offers flexibility in sample types, making the assay applicable to a wide range of research settings and experimental designs.
• Enhanced Understanding of Immune Responses: Contributes to a deeper understanding of the immune system′s complex interactions, particularly in the context of inflammatory diseases and other clinical conditions.
• Cardiovascular Risk Profiling: Analyze IFN-γ and MIP-1α, associated with atherosclerotic plaque instability to identify biomarkers for cardiovascular event prediction or therapeutic monitoring.
• Subpanel Flexibility: Select subsets of analytes (e.g., IL-2, IL-4, IL-5) for focused hypotheses, reducing costs and streamline workflows for targeted immune monitoring.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

For research use only. Not for use in diagnostic procedures.

Label License/Sticker for Assay Product:

By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.

Safety Information

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H302 + H312 + H332,H317,H318,H373,H411
• pcodes: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• target_organs: Respiratory Tract
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3