54457

HEPES

≥99.5% (titration), powder, for molecular biology, BioUltra

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• NACRES: NA.25
• PubChem Substance ID: 57651473
• UNSPSC Code: 12161700
• EC Number: 230-907-9
• MDL number: MFCD00006158
• Beilstein/REAXYS Number: 883043

Properties

• Product Name: HEPES, BioUltra, Molecular Biology, ≥99.5% (T)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• grade: Molecular Biology
• product line: BioUltra
• assay: ≥99.5% (T)
• form: powder
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• ign. residue: ≤0.1% (as SO₄)
• loss: ≤0.5% loss on drying, 110 °C
• pH: 5.0-6.5 (25 °C, 1 M in H₂O)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 1 M in H₂O
• UV absorption: λ: 260 nm A_max: 0.045; λ: 280 nm A_max: 0.035
• Quality Level: 200

Description

Application

HEPES has been used as a component of:

• DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells[1]
• RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)[2]
• Recapture solution to re-capture RNA exosomes from the glycerol gradient[3]
• ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation[4]
• 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors[5]

General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[6] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[7]

Other Notes

3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

Easily compare specifications for HEPES products with the HEPES specification table.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)