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Merck

A3553

Acrylamide

suitable for electrophoresis, ≥99% (HPLC), powder

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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€73.40

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€348.00

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€414.00

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About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
79-06-1
Molecular Weight:
71.08
NACRES:
NB.22
PubChem Substance ID:
24890783
eCl@ss:
39031205
UNSPSC Code:
41105319
EC Number:
201-173-7
MDL number:
MFCD00008032
Beilstein/REAXYS Number:
605349

Key Documents

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Product Name

Acrylamide, suitable for electrophoresis, ≥99% (HPLC), powder

cation traces

Fe: ≤1 ppm
Mg: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

form

powder

technique(s)

electrophoresis: suitable

impurities

≤0.005% Acrylic acid
≤0.005% Insoluble in water

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

Quality Level

200

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Acrylamide suitable for electrophoresis, ≥99% (HPLC), powder

Sigma-Aldrich

A3553

Acrylamide

Acrylamide Molecular Biology, ≥99% (HPLC)

Sigma-Aldrich

A9099

Acrylamide

Acrylamide for electrophoresis

Millipore

1.10784

Acrylamide

Acrylamide certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Supelco

08267

Acrylamide

form

powder

form

crystals

form

solid

form

-

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

impurities

≤0.005% Acrylic acid, ≤0.005% Insoluble in water

impurities

≤0.005% Acrylic acid content, ≤0.005% Water insolubles

impurities

-

impurities

-

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

-

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.009 hPa ( 25 °C)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

assay

≥99% (HPLC)

assay

-

assay

-

Application

Acrylamide has been used in the preparation of polyacrylamide gels.[1][2]

General description

Acrylamide functions as a quencher of tryptophan fluorescence.[3] Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels,[4] which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers.[4] Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis.[3]

Other Notes

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

used together

Product No.
Description
Pricing

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN9162
0000499023
0000496539
0000496539
0000499023

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Find documentation for the products that you have recently purchased in the Document Library.

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The Protein Protocols Handbook (2007)
Relationships among cell morphology, intrinsic cell stiffness and cell-substrate interactions
Chiang MYM, et al.
Biomaterials, 34(38), 9754-9762 (2013)
Chemistry, biochemistry, and safety of acrylamide. A review
Friedman M
Journal of Agricultural and Food Chemistry, 51(16), 4504-4526 (2003)
I-Chien Liao et al.
Advanced functional materials, 23(47), 5833-5839 (2014-03-01)
The development of synthetic biomaterials that possess mechanical properties that mimic those of native tissues remains an important challenge to the field of materials. In particular, articular cartilage is a complex nonlinear, viscoelastic, and anisotropic material that exhibits a very
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
View All Related Papers

Related Content

Product Information Sheet - A3553

Product Information Sheet

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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