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Merck

A9099

Acrylamide

Molecular Biology, ≥99% (HPLC)

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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25 G

€31.20

100 G

€94.60

500 G

€373.00

1 KG

€504.00

2.5 KG

€1,120.00

€31.20


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About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
Molecular Weight:
71.08
NACRES:
NA.31
PubChem Substance ID:
eCl@ss:
39031205
UNSPSC Code:
41105319
EC Number:
201-173-7
MDL number:
Beilstein/REAXYS Number:
605349

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Product Name

Acrylamide, Molecular Biology, ≥99% (HPLC)

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C

grade

Molecular Biology

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

assay

≥99% (HPLC)

form

crystals

impurities

≤0.005% Acrylic acid content
≤0.005% Water insolubles

conductivity

≤10 μmho (35% solution)

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

water: soluble 2.5 g/10 mL, clear, colorless

cation traces

Fe: ≤1 ppm
Mg: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

foreign activity

DNase, RNase, protease, none detected

Quality Level

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1 of 4

This Item
A3553A888701696
form

crystals

form

powder

form

crystals

form

powder blend

grade

for molecular biology

grade

-

grade

-

grade

for molecular biology

foreign activity

DNase, RNase, protease, none detected

foreign activity

-

foreign activity

-

foreign activity

-

impurities

≤0.005% Acrylic acid content

impurities

≤0.005% Acrylic acid, ≤0.005% Insoluble in water

impurities

-

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

vapor pressure

0.03 mmHg ( 40 °C)

Application

Acrylamide has been used:
  • in polyacrylamide gel electrophoresis[1][2]
  • in the preparation of cleared gel samples[3]
  • to determine the effect of L-cysteine on acrylamide-induced testicular toxicity[4]

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide. Acrylamide is a water-soluble monomer, which forms a transparent stable insoluble gel. Bis-acrylamide is a cross-linking agent.[5][6]

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Find documentation for the products that you have recently purchased in the Document Library.

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Separation of Thylakoid Protein Complexes with Two-dimensional Native-PAGE.
Rantala M, et al.
The Plant Journal, 8(13), 29089-29089 (2017)
Electrical characterization of DNA supported on nitrocellulose membranes.
Al AM, et al.
Scientific Reports, 6(4), 29089-29089 (2016)
Ana Techniqs in Biotechnology (2011)
Mild myelin disruption elicits early alteration in behavior and proliferation in the subventricular zone.
Gould EA,et al.
eLife, 7(4-5), e34783-e34783 (2018)
L-Cysteine Partially Protects Against Acrylamide-Induced Testicular Toxicity.
Kacar S, et al.
Balkan medical journal, 35(4), 311-311 (2018)

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Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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