G7017
β-Glucuronidase from Helix pomatia
Type HP-2, aqueous solution, ≥100,000 units/mL
β-Glucuronidase from Helix pomatia
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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1 ML
€65.30
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€120.00
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€247.00
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€442.00
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
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Product Name
β-Glucuronidase from Helix pomatia, Type HP-2, aqueous solution, ≥100,000 units/mL
biological source
Helix pomatia
type
Type HP-2
form
aqueous solution
specific activity
≥100,000 units/mL
secondary activity
≤7,500 units/mL sulfatase
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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Related Categories
1 of 4
This Item | G7770 | G0751 | G1512 |
|---|---|---|---|
| biological source Helix pomatia | biological source - | biological source - | biological source - |
| specific activity ≥100,000 units/mL | specific activity ≥90,000 units/mL | specific activity ≥300,000 units/g solid | specific activity ≥400,000 units/g solid |
| form aqueous solution | form aqueous solution | form partially purified powder | form lyophilized powder |
| shipped in wet ice | shipped in - | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Analysis Note
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
- Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
- Removal of glucuronide conjugates such as for the preparation of Baicalein.
Biochem/physiol Actions
Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.[1]
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.[1]
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Features and Benefits
- pH optima 4.0 - 5.0.[2]
- Sulfatase pH optima 6.2
- Low Sulfatase activity (<7,500 units/mL).
- Ammonium Sulfate suspension.
- Mollusk-derived beta-glucuronidase.
- Useful for acidic hydrolysis of glucuronides in low pH samples.
General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Similar to G0876, but produced by Sigma.
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Physical form
Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 1
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B De Roos et al.
Journal of internal medicine, 244(6), 451-460 (1999-01-20)
To determine the absorption and urinary excretion of the cholesterol-raising coffee diterpenes cafestol and kahweol in humans. Nine healthy ileostomists consumed a dose of one, two or three cups of French-press coffee together with a standardized breakfast on three separate
W Dennis Ulrey et al.
Journal of AOAC International, 96(4), 917-924 (2013-09-05)
A multilaboratory study of AOAC Official Method 2011.23 was performed to satisfy requirements for Final Action status through the AOAC expert review panel process. The study included nine collaborating laboratories from the United States, Canada, Brazil, and The Netherlands. Five
L Bláhová et al.
Journal of dairy science, 99(9), 6973-6982 (2016-07-11)
Isoflavones are natural phytoestrogens with antioxidant and endocrine-disrupting potencies. Monitoring of their levels is important to ensure the high quality and safety of food, milk, and dairy products. The efficiency and accuracy of phytoestrogen analyses in complex matrices such as
Mladen Boban et al.
Journal of cardiovascular pharmacology, 47(5), 695-701 (2006-06-16)
By using red wine (RW), dealcoholized red wine (DARW), polyphenols-stripped red wine (PSRW), ethanol-water solution (ET), and water (W), the role of wine polyphenols, ethanol, and urate on vascular function was examined in humans (n = 9 per beverage) and
Jack R T Darby et al.
The Journal of physiology, 597(20), 5063-5077 (2019-09-05)
Substrate restriction during critical developmental windows of gestation programmes offspring for a predisposition towards cardiovascular disease in adult life. This study aimed to determine the effect of maternal resveratrol (RSV) treatment in an animal model in which chronic fetal catheterisation
Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Protocols
Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.
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