G7396
β-Glucuronidase from Escherichia coli
Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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€76.80
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Product Name
β-Glucuronidase from Escherichia coli, Type IX-A, lyophilized powder, 1,000,000-5,000,000 units/g protein (30 min assay)
type
Type IX-A
form
lyophilized powder
specific activity
1,000,000-5,000,000 units/g protein (30 min assay)
mol wt
290 kDa
composition
Protein, 30-70% biuret
shipped in
wet ice
storage temp.
−20°C
Quality Level
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Related Categories
1 of 4
This Item | G8295 | G8420 | G8162 |
|---|---|---|---|
| specific activity 1,000,000-5,000,000 units/g protein (30 min assay) | specific activity ≥10,000,000 units/g protein (30 min assay) | specific activity ≥20,000 units/mg protein | specific activity ≥5,000,000 units/g protein, pH 6.8 (biuret) |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form aqueous glycerol solution |
| mol wt 290 kDa | mol wt ~290 kDa | mol wt 290 kDa | mol wt 69-71 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in wet ice | shipped in - | shipped in - | shipped in wet ice |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
Analysis Note
Application
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Other Notes
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.
Protocols
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.
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