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Merck

I3390

IMDM

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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500 ML

€46.60

1 L

€75.90

€46.60

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217469

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Product Name

Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: yes
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

Quality Level

400

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This Item
51471CI6529I7633
Sigma-Aldrich

Sigma-Aldrich

I3390

Iscove′s Modified Dulbecco′s Medium

Iscove’s Modified Dulbecco’s Medium liquid

SAFC

51471C

Iscove′s Modified Dulbecco′s Medium

SAFC

SAFC

I6529

Iscove′s Modified Dulbecco′s Medium

Iscove’s Modified Dulbecco’s Medium With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

I7633

Iscove′s Modified Dulbecco′s Medium

sterility

sterile-filtered

sterility

sterile

sterility

sterile-filtered

sterility

-

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

powder

components

phenol red: yes
L-glutamine: no
glucose: yes

components

phenol red: 15.93 mg/L
NaHCO3: 3024 mg/L
L-glutamine: 582 mg/L
HEPES: 5958 mg/L
sodium pyruvate: 110 mg/L

components

glucose: yes
phenol red: yes
NaHCO3: yes
L-glutamine: yes

components

phenol red: yes
glucose: yes
L-glutamine: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Preparation Note

Supplement with 0.584 g/L L-glutamine

related product

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Description
Pricing

used together

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Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0971
RNBL9599
RNBL9388
RNBL6331
RNBL4925

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N N Iscove et al.
The Journal of experimental medicine, 147(3), 923-933 (1978-03-01)
Albumin, transferrin, and lipids can replace serum entirely for support of LPS-stimulated murine B lymphocytes in culture. In the presence of these compounds, growth and maturation to IgM and IgG secretion, induced by lipopolysaccharide (LPS), occurs at the same or
N Vriezen et al.
Biotechnology and bioengineering, 54(3), 272-286 (1997-05-05)
Glutamine is a major source of energy, carbon, and nitrogen for mammalian cells. The amount of glutamine present in commercial mammalian cell media is, however, not necessarily balanced with cell requirements. Therefore, the effects of glutamine limitation on the physiology
Sophie G Kellaway et al.
Haematologica, 106(6), 1569-1580 (2020-04-18)
Hematological malignancies are characterised by a block in differentiation, which in many cases is caused by recurrent mutations affecting the activity of hematopoietic transcription factors. RUNX1-EVI1 is a fusion protein formed by the t(3;21) translocation linking two transcription factors required
Kirsten Gillingwater
Parasitology, 145(7), 953-960 (2017-11-24)
Trypanosoma equiperdum is a protozoan parasite responsible for causing Dourine, a debilitating neglected veterinary disease, found worldwide affecting equids. It is the only pathogenic trypanosome species that does not require an invertebrate vector for transmission, thus being passed from animal
Dillon M Voss et al.
Cancer research, 80(21), 4681-4692 (2020-09-16)
Muscleblind-like proteins (MBNL) belong to a family of tissue-specific regulators of RNA metabolism that control premessenger RNA splicing. Inactivation of MBNL causes an adult-to-fetal alternative splicing transition, resulting in the development of myotonic dystrophy. We have previously shown that the
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