The solubility of this material is tested in DMSO. The solubility in water has not been determined. However, external sources indicate that Pyridostatin hydrochloride is soluble in water at 1 mM. Solutions may be stored up to 1 month at -20°C (store solutions under nitrogen). The HCl content varies from lot to lot and may range from 4.5 to 5.5 mol/mol. The water content may also range from 0.0 - 7.0 mol/mol. This information is reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/sml2690#product-documentation
SML2690
Pyridostatin hydrochloride
≥98% (HPLC), powder, G-quadruplex DNA stabilizing agent
Pyridostatin hydrochloride
Synonym(s):
4-(2-Aminoethoxy)-N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)pyridine-2,6-dicarboxamide hydrochloride, 4-(2-Aminoethoxy)-N2,N6-bis[4-(2-aminoethoxy)-2-quinolinyl]-2,6-pyridinedicarboxamide hydrochloride, RR 82 HCl, RR-82 HCl, RR82 HCl
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5 MG
€104.00
25 MG
€421.00
About This Item
Empirical Formula (Hill Notation):
C31H32N8O5 · xHCl
Molecular Weight:
596.64 (free base basis)
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
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Product Name
Pyridostatin hydrochloride, ≥98% (HPLC)
SMILES string
NCCOc1cc(nc(c1)C(=O)Nc4nc5c(c(c4)OCCN)cccc5)C(=O)Nc2nc3c(c(c2)OCCN)cccc3
InChI key
VGHSATQVJCTKEF-UHFFFAOYSA-N
assay
≥98% (HPLC)
form
powder
storage condition
desiccated
color
white to beige
solubility
DMSO: 2 mg/mL, clear
storage temp.
2-8°C
Related Categories
1 of 4
This Item | SML2921 | SML2436 | SML0952 |
|---|---|---|---|
| form powder | form powder | form powder | form powder |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay ≥97% (HPLC) |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| storage condition desiccated | storage condition desiccated | storage condition - | storage condition desiccated |
| solubility DMSO: 2 mg/mL, clear | solubility DMSO: 2 mg/mL, clear | solubility DMSO: 2 mg/mL, clear | solubility H2O: 10 mg/mL, clear |
| color white to beige | color white to beige | color white to beige | color white to beige |
Application
Pyridostatin hydrochloride has been used as a G-quadruplex (G4)-stabilizing ligand to check the effect of G4s on fork progression. It has also been used as a G4-stabilizing ligand to study the effect of G4 on apurinic/apyrimidinic endonuclease 1 (APE1) mediated Kirsten rat sarcoma virus (KRAS) expression in pancreatic ductal adenocarcinoma (PDAC) cell lines.
Biochem/physiol Actions
Pyridostatin is a highly selective G-quadruplex (G4) interacting molecule. It retards the growth of human cancer cells by inducing replication/transcription dependent on DNA damage. Pyridostatin stabilizes G-quadruplexes, targeting the proto-oncogene SRC and telomeric G4, inducing DNA damage and cell-cycle arrest.
Stabilizer of G-quadruplexes (G4).
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hong-Liang Bao et al.
Nature protocols, 13(4), 652-665 (2018-03-09)
Growing evidence indicates that RNA G-quadruplexes have important roles in various processes such as transcription, translation, regulation of telomere length, and formation of telomeric heterochromatin. Investigation of RNA G-quadruplex structures associated with biological events is therefore essential to understanding the
Kaitlin Johnson et al.
RNA (New York, N.Y.), 30(9), 1213-1226 (2024-06-26)
Telomere replication is essential for continued proliferation of human cells, such as stem cells and cancer cells. Telomerase lengthens the telomeric G-strand, while C-strand replication is accomplished by CST-polymerase α-primase (CST-PP). Replication of both strands is inhibited by formation of
Charanya Kumar et al.
eLife, 10 (2021-09-09)
R-loops are a major source of genome instability associated with transcription-induced replication stress. However, how R-loops inherently impact replication fork progression is not understood. Here, we characterize R-loop-replisome collisions using a fully reconstituted eukaryotic DNA replication system. We find that
Alessio De Magis et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(3), 816-825 (2018-12-29)
G quadruplexes (G4s) and R loops are noncanonical DNA structures that can regulate basic nuclear processes and trigger DNA damage, genome instability, and cell killing. By different technical approaches, we here establish that specific G4 ligands stabilize G4s and simultaneously
Suravi Pramanik et al.
Nucleic acids research, 50(6), 3394-3412 (2022-03-15)
Pancreatic ductal adenocarcinoma (PDAC), one of the most aggressive types of cancer, is characterized by aberrant activity of oncogenic KRAS. A nuclease-hypersensitive GC-rich region in KRAS promoter can fold into a four-stranded DNA secondary structure called G-quadruplex (G4), known to
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Hi, I need to make up a known concentration solution of this compound, but you only supply a free base molecular weight. Can you provide me with a full molecular weight for lot #0000374186? Also, what's the storage and water solubility? Thanks in advance
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