The gelation process for this product does not require pH neutralization by the addition of acid or base. The procedure involves pipetting undiluted collagen into culture dishes, exposing them to ammonia vapor, and rinsing with sterile distilled water multiple times to remove ammonia. The final rinse is replaced with a sterile medium or balanced salt solution with serum, and the dishes are stored at 4°C until use.
08-115
Rat Collagen Type I
from rat tail, liquid, 2-5 mg/mL, suitable for cell culture, used for 3D gel formation
Synonyme(s) :
Collagène de queue de rat, Collagène de type I de queue de rat
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Sélectionner une taille de conditionnement
100 MG
352,00 €
A propos de cet article
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.75
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Collagène de type I, queue de rat, 100 mg, solution, BioReagent, suitable for cell culture
Source biologique
rat
Niveau de qualité
Forme
liquid
solution
Fabricant/nom de marque
Upstate®
Concentration
100 mg
Technique(s)
cell culture | mammalian: suitable (and for 3D proliferation assays.)
Entrée
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Informations sur le gène
rat ... COL1A1(29393)
1 of 4
Cet article | 11179179001 | C3867 | 234138 |
|---|---|---|---|
| biological source rat | biological source rat tail | biological source rat tail | biological source human skin |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form liquid | form lyophilized (clear, colorless solution after reconstitution) | form liquid | form lyophilized |
| shipped in wet ice | shipped in - | shipped in wet ice | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
Description générale
Chez l'homme, le collagène de type I est le collagène le plus abondant et le plus largement exprimé. Cet hétérotrimère contient deux chaînes alpha 1 (I) et une chaîne alpha 2 (I).[1] Le collagène de type I α 1 (COL1A1), un composant majeur du collagène de type I, code pour les chaînes pro-α 1 du collagène de type I.[2] Il contient des motifs tri-peptidiques répétés Gly-Xaa-Yaa. La protéine de la chaîne alpha-1(I) du collagène est un membre de la superfamille du complexe de collagène.[1] COL1A1 possède un domaine de facteur de von Willebrand de type C (vWFC), une répétition de triple hélice de collagène et un domaine C-terminal de collagène fibrillaire (COLF).[2]
Application
Le collagène de type I issu de queue de rat a été utilisé :
- pour les tests de prolifération du collagène en 3D[1]
- pour les tests de prolifération de l'atelocollagène[1]
- dans une étude comparative visant à fabriquer des microcapsules pouvant servir de modèles de noyaux mésoporeux de cristaux purs de carbonate de calcium (CaCO3) sous sa forme vatérite, afin d'évaluer les relations structure-propriétés[2]
- pour le revêtement de lamelles pour cultiver la lignée de cellules endothéliales micro-vasculaires cérébrales humaines (hCMEC/D3) afin de créer une co-culture cellulaire et un modèle de lésion induite à partir d'un échantillon issu d'un patient atteint d'hématome sous-dural chronique (CSDH).[3]
Actions biochimiques/physiologiques
Le collagène de type I α 1 (COL1A1) joue un rôle clé dans la résistance aux chimiothérapies et pourrait servir de cible thérapeutique. Il joue un rôle dans les cancers ovariens (OV) résistants au carboplatine.[1] COL1A1 sert d'oncogène dans le développement des cancers du côlon et du rectum (CRC).[2] Les mutations héréditaires dominantes du gène COL1A1 entraînent des troubles des tissus conjonctifs tels que l'ostéogenèse imparfaite (OI).[1]
Forme physique
Acide acétique 0,02 N, pH 3,67
Notes préparatoires
1 an à 4 °C
Remarque sur l'analyse
Produit évalué en routine en testant la présence de gélification uniforme et l'adhésion et l'expansion de cellules PC-12 de rat.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Sauf indication contraire dans notre catalogue ou toute autre documentation associée au(x) produit(s), nos produits sont uniquement destinés à la recherche et ne sauraient être utilisés à d'autres fins, ce qui inclut, sans s'y limiter, les utilisations commerciales non autorisées, les utilisations diagnostiques in vitro, les utilisations thérapeutiques ex vivo ou in vivo, ou tout type de consommation ou d'application chez l'être humain ou chez l'animal.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
M Yebra et al.
The Journal of biological chemistry, 271(46), 29393-29399 (1996-11-15)
The urokinase plasminogen activator (uPA) interacts with its cell surface receptor (uPAR), providing an inducible, localized cell surface proteolytic activity, thereby promoting cellular invasion. Evidence is provided for a novel function of cell surface-associated uPA.uPAR. Specifically, induction of cell surface
Kerim B Kaylan et al.
Scientific reports, 6, 23490-23490 (2016-03-31)
The bipotential differentiation of liver progenitor cells underlies liver development and bile duct formation as well as liver regeneration and disease. TGFβ and Notch signaling are known to play important roles in the liver progenitor specification process and tissue morphogenesis.
C Fink et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(5), 669-679 (2000-04-01)
To examine the influence of chronic mechanical stretch on functional behavior of cardiac myocytes, we reconstituted embryonic chick or neonatal rat cardiac myocytes to a 3-dimensional engineered heart tissue (EHT) by mixing freshly isolated cells with neutralized collagen I and
J J Tomasek et al.
The Journal of biological chemistry, 272(11), 7482-7487 (1997-03-14)
Gelatinase A (GL-A) is a matrix metalloproteinase (MMP) involved in both connective tissue remodeling and tumor invasion. GL-A activation is mediated by a membrane-type MMP (MT-MMP) that cleaves the GL-A propeptide. In this study, we examined the role of the
H Uyttendaele et al.
Developmental biology, 196(2), 204-217 (1998-05-13)
Elongation and branching of epithelial ducts is a crucial event during the development of the mammary gland. Branching morphogenesis of the mouse mammary epithelial TAC-2 cell line was used as an assay to examine the role of Wnt, HGF, TGF-beta
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Does this product require pH neutralization by addition of acid or base for gelation?
1 answer-
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Hi team: I hope this finds you well. May I know the concentration of collagen type I , rat tail 08-115? Much appreciated. Kind regards, Ovi
1 answer-
The concentration is provided in lot specific Certificates of Analysis, typically within the range of 3 to 5 mg/ml.
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Accidentally, I opened the collagen out of the flow chamber. Should I filter it or autoclave it to ensure the sterile conditions?
1 answer-
Collagen solutions may be sterilized by dialysis in a 0.25% acetic acid and 0.5% chloroform solution.
Avoid sterilizing the collagen solution by membrane filtration, as substantial protein loss may occur by this method. The coating of tissue culture plastic dishes may be performed by air-drying. Dried coated dishes may then be sterilized by exposure to UV light in a sterile culture hood or by rinsing with 70% ethanol.Helpful?
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Hi, what is the concentration of the collagen?
1 answer-
The concentration of this product will be listed on the lot-specific Certificate of Analysis. Please see the link below to access a Certificate:
https://www.emdmillipore.com/US/en/product/Collagen-Type-I-rat-tail,MM_NF-08-115#anchor_COAHelpful?
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Once diluted in 0.02N acetic acid, does the collagen solution require sterilisation for use in cell culture? If so, which method is the most effective: UV, autoclave or 0.22uM sterile filter (should this be PES or PVDF)?
1 answer-
This product is supplied as a sterile solution in 0.02 N Acetic acid. The package size is 100 mg, however, the concentration is variable and reported on the lot-specific Certificate of Analysis. The typical range is 4 - 5 mg/mL. Dilutions can be made with sterile PBS. Further sterilization is not required. If necessary, coated slides and coverslips can be sterilized by UV.
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