1.11699

Differential reinforced clostridial broth (DRCM)

GranuCult^® plus, according to DIN Norm 38411, ISO 6461, granular, selective for Clostridium spp., pack of 500 g

• Synonyme(s) : Clostridium differential broth, DRCM
• Code UNSPSC : 41106213
• Nomenclature NACRES : NA.74

Propriétés

• Agence: ISO 6461; according to DIN Norm 38411
• Niveau de qualité: 200
• Stérilité: non-sterile
• Forme: granular
• Conditionnement: pack of 500 g
• Fabricant/nom de marque: GranuCult^® plus
• Conditions de stockage: dry media (Keep tightly closed)
• Technique(s): microbiological culture: suitable
• Couleur: brown
• pH: 7.0-7.2 (25 °C, 28 g/L in H₂O, after autoclaving)
• Solubilité: 27.5 g/L
• Masse volumique apparente: 650 kg/m³
• Application(s): food and beverages; microbiology
• Température de stockage: 15-25°C
• Adéquation: selective for Clostridium spp.

Description

Description générale

Differential Reinforced Clostridial Broth is used to determine the count of sulfite-reducing bacteria by the MPN technique. The redox indicator resazurin is used to monitor anaerobiosis. Clostridia reduce sulfite to sulfide, which causes the culture medium to turn black. As other bacteria can also produce sulfide, vegetative forms must first be removed from the culture by a relevant treatment (e.g., pasteurization), and the anaerobic spore-forming microorganisms must then be identified. The addition of polymyxin (70 IU/ml) to the broth inhibits the growth of most non-spore-forming microorganisms.

Application

Differential reinforced clostridial broth is suitable for the enumeration of all clostridia by the MPN method in foodstuffs and other materials.

Caractéristiques et avantages

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Remarque sur l'analyse

Appearance (clearness): clear
Appearance (colour): reddish-brown
pH-value (25 °C): 6.9 - 7.3
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Bacillus cereus ATCC 11778 (WDCM 00001)): fair to good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): poor to fair
Growth (Clostridium bifermentans ATCC 19299): good to very good
Growth (Clostridium perfringens ATCC 13124 (WDCM 00007)): good to very good
Growth (Clostridium perfringens ATCC 10543): good to very good
Growth (Clostridium sporogenes ATCC 11437): good to very good
Growth (Clostridium sporogenes ATCC 19404 (WDCM 00008)): good to very good
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Bacillus cereus ATCC 11778 (WDCM 00001)): -
Blacking (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Blacking (Clostridium bifermentans ATCC 19299): +
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007)): +
Blacking (Clostridium perfringens ATCC 10543): +
Blacking (Clostridium sporogenes ATCC 11437): +
Blacking (Clostridium sporogenes ATCC 19404 (WDCM 00008)): +
Incubation: 7 days; 30 °C; layered with paraffin.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H319
• Conseils de prudence: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classification des risques: Eye Irrit. 2
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3