FCMAB186F

Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1

clone B-Ly1, Milli-Mark^®, from mouse

• Synonyme(s) : Membrane-spanning 4-domains subfamily A member 1 ; ; B-lymphocyte surface antigen B1 ; ; Leukocyte surface antigen Leu-16 ; ; Bp35 ; ; CD_antigen=CD20
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: FITC conjugate
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: B-Ly1, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Milli-Mark^®
• Technique(s): flow cytometry: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_690605.1
• Numéro d'accès UniProt: P11836
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MS4A1(931)

Description

Description générale

33 kDa (Zhou, 1995)

CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells (Tedder, 1994). In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines (Zhou, 1995). The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface (Tedder, 1994). Antibodies reacting with CD20 cytoplasmic epitopes are designated CD20cy (Zhou, 1995). It is suggested that CD20 plays a direct role in regulating the transmembrane conductive Ca 2+ flux of B cells which indicates a possible function for CD20 as a regulator of proliferation and differentiation (Tedder, 1994).

Application

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Milli-Mark Anti-CD20 -FITC Antibody, clone B-Ly1 is validated for use in FC for the detection of CD20.

Actions biochimiques/physiologiques

Antibody recognizes Human CD20 antigen.

Forme physique

Protein A purified

Purified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2

Notes préparatoires

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Remarque sur l'analyse

Control
Human Lymphocytes

Evaluated by flow cytometry using human lymphocytes.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable