MABC602

Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2

clone 1-2.3.1.2, from mouse

• Synonyme(s) : Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein, Ferritin heavy chain, N-terminally processed
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 1-2.3.1.2, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_002023
• Numéro d'accès UniProt: P02794
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FTH1(2495)

Description

Description générale

Ferritin is a widely expressed, very large, multi-subunit, intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms in some form. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin protein is a multi-subunit (24 oligomers) protein complex consisting of both large (heavy) and small (light) chains. It is primarily and intracellular protein.The subunits of Ferritin in vertebrates have an apparent molecular weight of 19 kDa or 21 kDa respectively; their sequences are about 50% homologous. When Ferritin is unbound by iron the protein is called Apoferritin. Mutations associated with the Ferritin Heavy Chain protein contribute to a syndrome called Hemochromatosis 5 which is a disorder of iron metabolism where too much iron is retained within the tissues resulting in a variety of syndromes which unfortunately manifest themselves late in life.

~21 kDa observed. Uncharacterized band may be observed at ~200 kDa

Immunogène

Recombinant protein corresponding to human Ferritin Heavy Chain.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Ferritin Heavy Chain in human bone marrow and human tonsil tissue.
Immunohistochemistry Analysis: A representative lot detected Ferritin Heavy Chain in erythroid blasts of acute myeloid leukaemia M6 tissue, macrophages in the lymph node tissue, and tumor & lung-associated macrophages (Wang, W., et al. (2013). Histopahtology. 62:931–940).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Ferritin Heavy Chain Antibody, clone 1-2.3.1.2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Ferritin Heavy Chain.

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Notes préparatoires

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Evaluated by Western Blotting in U251 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ferritin Heavy Chain in 10 µg of U251 cell lysate.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable