Methylene Blue solution

Name: Methylene Blue solution
Brand: SIGMA
Price: 41.6 EUR

concentrate according to Ehrlich, concentrated, aqueous solution

Synonyme(s) :

Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue

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250 ML

41,60 €

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A propos de cet article

Formule empirique (notation de Hill) :

C₁₆H₁₈ClN₃S

Numéro CAS:

61-73-4

Poids moléculaire :

319.85

NACRES:

NA.47

PubChem Substance ID:

329747853

UNSPSC Code:

12171500

MDL number:

MFCD00012111

Beilstein/REAXYS Number:

3641570

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Nom du produit

Methylene Blue solution, concentrate according to Ehrlich, concentrated, aqueous solution

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

form

liquid

quality

concentrated, aqueous solution

shelf life

~3 yr

concentration

1.5% (1.5 g/ 100 mL)

density

1.0 g/mL at 20 °C

ε_max

9.4 at 656-666 nm in water

suitability

suitable for microscopy

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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Catégories apparentées

Clinical & Diagnostics

Hematology Stains

Tissue Diagnostics

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1 of 4

Cet article	319112	457250	Y0001744
Sigma-Aldrich 03978 Methylene Blue solution	Sigma-Aldrich 319112 Methylene Blue solution	Sigma-Aldrich 457250 Methylene Blue	Y0001744 Methylthioninium for system suitability
grade for microscopy	grade -	grade -	grade pharmaceutical primary standard
concentration 1.5% (1.5 g/ 100 mL)	concentration 0.05 wt. % in H₂O	concentration -	concentration -
form liquid	form liquid	form solid	form -
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) -	application(s) pharmaceutical (small molecule)
shelf life ~3 yr	shelf life -	shelf life -	shelf life -
storage temp. room temp	storage temp. room temp	storage temp. 10-30°C	storage temp. 2-8°C

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability.[1] It has also been used to stain sections to determine the presence of mycobacteria.[2][3]

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

General description

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye.[4] It is a small molecular weight dye,[5] which is effective against Gram positive and Gram negative bacteria.[6]

Classe de stockage

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Real-time fluorescence image-guided oncologic surgery

Advances in Cancer Research (2014)

Interferon-gamma is a crucial activator of early host immune defense against Mycobacterium ulcerans infection in mice

Bieri R, et al.

PLoS Neglected Tropical Diseases, 10(2), e0004450-e0004450 (2016)

Antimicrobial Photodynamic Therapy (APDT) Action Based on Nanostructured Photosensitizers

Multifunctional Systems for Combined Delivery Biosensing and Diagnostics (2017)

CLEC9A modulates macrophage-mediated neutrophil recruitment in response to heat-killed Mycobacterium tuberculosis H37Ra

Cheng An-Chieh, et al.

Testing, 12(10), e0186780-e0186780 (2017)

Reprogramming human amniotic fluid stem cells to functional pluripotency by manipulation of culture conditions

Moschidou D and Guillot PV

National Protocol Exchange (2012)

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Questions

Anonymous

a year ago

Concerning the stated concentration of the product 03978 (Methylene blue solution, 1.5%), what is the uncertainty and lot-to-lot variability?

1 answer
1. Technical Support
  
  a year ago
  
  Product 03978 is not a GMP quality product. No testing is performed during Quality Control testing that measures lot to lot variability. The testing includes tests for Appearance, Thin Layer Chromatography, Density, Wavelength 1 at 656-666nm, Extinction Coefficient for Wavelength 1 and the solvent used to measure the wavelength and extinction coefficient. The information about a 1.5% concentration of dye is from the production documents used to manufacture the dye solution. The actual dye concentration is not measured.
  
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