It is not possible to have cells that are PI positive without the cells also being positive for Annexin V binding. Cells are PI positive because the membrane has been compromised. If this is the case, Annexin V can also enter the cell and bind to the PS on the internal cell membrane. The gating on the histogram for the FITC channel should be changed so that all cells that are PI positive are also Annexin V positive.
APOAF
Annexin V-FITC Apoptosis Detection Kit
Synonyme(s) :
Annexin V-FITC, Apoptosis probe FITC
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20 TEST
325,00 €
60 TEST
699,00 €
325,00 €
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A propos de cet article
NACRES:
NA.84
UNSPSC Code:
12352207
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(20 tests)
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Quality Level
1 of 4
Cet article | APOAC | S7111 | QIA39 |
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable | technique(s) flow cytometry: suitable |
| usage (20 tests) | usage sufficient for 200 tests | usage - | usage sufficient for 50 tests |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging - |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) - |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
Application
Annexin V-FITC Apoptosis Detection Kit was used:
- in staining of LNCaP prostate cancer cells for measuring the G. lucidum extracts activity during the treatment of prostate cancer.[1]
- for tumor cell labelling to study the inhibitory activity of DBP-maf (Vitamin D binding protein-macrophage activating factor) on prostate tumor cells.[2]
- for indirect measurement of flippase activity.[3]
Features and Benefits
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
- Rapid labeling of cells. Cell staining takes only 10 minutes.
- No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
- Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.
General description
Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).
Other Notes
Allow all components to reach room temperature before use.
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Description
Tarif
Classe de stockage
10 - Combustible liquids
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Ben-Zion Zaidman et al.
International journal of oncology, 31(4), 959-967 (2007-09-06)
Ganoderma lucidum (Curt.:Fr.) P. Karst, a medicinal fungus, has been widely used in Asian countries for centuries to prevent or treat a variety of diseases, including cancer. However, the mechanisms responsible for the effects of G. lucidum on cancer cells
Juliana Rizzo et al.
Eukaryotic cell, 13(6), 715-726 (2013-12-18)
Flippases are key regulators of membrane asymmetry and secretory mechanisms. Vesicular polysaccharide secretion is essential for the pathogenic mechanisms of Cryptococcus neoformans. On the basis of the observations that flippases are required for polysaccharide secretion in plants and the putative
Denise C Arruda et al.
The Journal of biological chemistry, 287(18), 14912-14922 (2012-02-16)
Complementarity-determining regions (CDRs) from monoclonal antibodies tested as synthetic peptides display anti-infective and antitumor activities, independent of the specificity of the native antibody. Previously, we have shown that the synthetic peptide C7H2, based on the heavy chain CDR 2 from
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Our study focus on the biological importance of synthesized 5β-dihydrocortisol (Dhc) and 5β-dihydrocortisol acetate (DhcA) molecules, the cytotoxic study was performed on breast cancer cell line (MCF-7) normal human embryonic kidney cell line (HEK293), the IC50 values for MCF-7 cells
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Metformin, one of the most common prescriptions for patients with type 2 diabetes, is reported to protect the kidney from gentamicin-induced nephrotoxicity. However, the role and mechanisms for metformin in preventing cisplatin-induced nephrotoxicity remains largely unknown. In this study, a
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Contenu apparenté
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When using Product APOAF, Annexin V-FITC Apoptosis Detection Kit, I have cells that are Annexin V FITC negative and PI positive. What are these cells?
1 answer-
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Can I use Product APOAF, Annexin V-FITC Apoptosis Detection Kit, to differentiate cells that are dead due to necrosis or apoptosis?
1 answer-
When using a kinetic study (various time points), you can show the progression of the cells from viable (annexin V FITC negative, PI negative), to annexin FITC positive, PI negative (membrane flip) to annexin V FITC positive, PI positive (dead). If there are cells that are double positive when starting, it is not possible to guarantee that the cell death occurred due to apoptosis with this assay.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Product APOAF, Annexin V-FITC Apoptosis Detection Kit be used on fixed cells?
1 answer-
No. Product APOAF, Annexin V-FITC Apoptosis Detection Kit must be performed on live cells in order to measure Apoptosis. The assay is based on the externization of phosphatidylserine from the inner cell membrane to the outer cell membrane. If the membrane is preturbed due to fixation, non-speciifc staining of the inner cell membrane might occur
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When using the Annexin V-FITC Apoptosis Detection Kit, Product APOAF, can I use any buffer for resuspending my cells?
1 answer-
The binding buffer included in the kit needs to be used for resuspending cells. The buffer contains calcium chloride at a final (1X) concentration of 2.5 mM which is necessary for the binding of annexin V to phosphatidylserine.
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What wavelength do I use to detect Annexin V-FITC and Propidium Iodide when using Annexin V-FITC Apoptosis Detection Kit, Product APOAF?
1 answer-
Annexin V FITC will have a maximum emission of 528 nm. This can be measured in the standard FITC Channel on a flow cytometer (FL1). Propidium Iodide has a maximum emission of 620 nm. This is measured on the short red channel on a flow cytometer (FL2 or FL3).
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