This product is a liquid medium. The density is not reported. The 1L volume weighs approximately 1 kilogram. The total weight of the packaged 1L size is 1.1 kg. The total weight of the packaged 500 mL size is 578 g.
D6429
DMEM - high glucose
With sodium bicarbonate, ʟ-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Modified Eagle’s Medium - high glucose
1 of 5 reviewers received a sample product or took part in a promotion
Synonyme(s) :
DME, DMEM
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500 ML
32,80 €
1 L
62,40 €
6 X 500 ML
195,00 €
24 X 500 ML
614,00 €
32,80 €
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A propos de cet article
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.71
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Laissez-nous vous aiderNom du produit
Milieu d′Eagle modifié par Dulbecco (DMEM) à teneur élevée en glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Niveau de qualité
Stérilité
sterile-filtered
Forme
liquid
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Composants
HEPES: no
NaHCO3: yes
phenol red: yes
sodium pyruvate: yes
L-glutamine: yes
glucose: high
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Cet article | D6546 | D5796 | D1145 |
|---|---|---|---|
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form liquid | form liquid | form liquid | form liquid |
| components HEPES: no | components NaHCO3: yes | components NaHCO3: yes | components L-glutamine: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
Description générale
Ce milieu DMEM à teneur élevée en glucose ("DMEM-Hi") est un milieu complet 1x additionné de pyruvate de sodium. Il diffère également de la formulation DMEM-Hi d'origine en ce que le pyridoxal a été remplacé par de la pyridoxine. Le pyridoxal est un constituant instable des milieux.
Application
Le Milieu de Eagle modifié par Dulbecco (DMEM) à teneur élevée en glucose est utilisé par la culture de cellules.[1][2][3]
Le milieu de Eagle modifié par Dulbecco (DMEM) est une variante du milieu de Eagle de base (BME) qui contient quatre fois plus d'acides aminés et de vitamines. La formulation d'origine contenait 1 000 mg/l de glucose et servait à la culture de cellules embryonnaires de souris. Depuis, le milieu a été modifié à plusieurs reprises pour convenir aux primocultures de cellules de souris et de poulet ainsi que de diverses cellules normales ou transformées. Chacun de ces milieux apporte une combinaison différente de L-glutamine et de pyruvate de sodium. En outre, le taux de glucose a été augmenté à 4 500 mg/l, d'où le nom "DMEM/High" pour DMEM riche en glucose.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Instructions
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How much does D6429-1L weigh? Thank you!
1 answer-
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Hi, What is the concentration of endotoxin detected in DMEM high glucose media D6429?
1 answer-
This product has an endotoxin specification of ≤ 1 EU/ml or 0.1 ng/mL. The lot specific values are reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/d6429#product-documentationHelpful?
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What is the concentration of Sodium Bicarbonate and Sodium Pyruvate in it?
1 answer-
This formulation contains 3.7 g/L sodium bicarbonate and 110 mg/L sodium pyruvate. The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
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After opening a bottle of medium: how long can I use this bottle ?(stored at 4C°)
1 answer-
Provided that the product is handled and stored properly and has not been contaminated, the medium will be stable up to the expiration date. This date is provided on the label and the lot-specific Certificate of Analysis.
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I would like to inquire about the amino acid formulation in DMEM (#6429, #6546). Specifically, could you provide information on the concentration of aspartate and glutamate in these formulations? Thank you very much.
1 answer-
The composition of this product is published on page 3 of the product information sheet. Please see the link below to access this document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/318/256/d6429for.pdf
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