This product has an osmolarity range of 275–304 mOsm/kg.
D8537
Solution saline dans un tampon phosphate de Dulbecco
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Phosphate Buffered Saline
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Synonyme(s) :
DPBS
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100 ML
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Solution saline dans un tampon phosphate de Dulbecco, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Niveau de qualité
Stérilité
sterile-filtered
Forme
liquid
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin, tested
Composants
glucose: no (Dextro)
sodium pyruvate: no
Conditions d'expédition
ambient
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Cet article | 59321C | 59331C | 59300C |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 300 | Quality Level 400 | Quality Level 400 | Quality Level 500 |
| form liquid | form liquid | form liquid | form liquid |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| impurities endotoxin, tested | impurities - | impurities - | impurities - |
d-PBS, Dulbeccos PBS, Dulbeccos Buffer, Phosphate Buffered Saline, buffered saline Dulbeccos, balanced salt solution (BSS), bss for mammalian cells, | Isotonic saline, cell washing solution, cell suspension solution | Isotonic saline, cell washing solution, cell suspension solution | DPBS, Isotonic saline, cell washing solution, cell suspension solution |
Description générale
La solution saline tamponnée au phosphate de Dulbecco (DPBS) est une solution saline équilibrée (BSS, Balanced Salt Solution) utilisée pour la manipulation et la culture des cellules de mammifère. Le tampon DPBS est utilisé pour irriguer, laver et diluer les cellules de mammifère tout en maintenant l'équilibre osmotique. Il apporte l'eau et les ions inorganiques essentiels au métabolisme normal des cellules. Le DPBS additionné de calcium et de magnésium facilite la liaison et l'agrégation cellulaires. Le PBS de Dulbecco sans ajout de ces ions peut être utilisé pour laver et rincer des cellules en suspension. Le tamponnage au phosphate maintient le pH dans la plage physiologique.
Application
La solution saline tamponnée au phosphate de Dulbecco (DPBS) a été utilisée :
- pour la dilution de globules rouges frais afin d'étudier le piégeage d'agrégats de globules rouges.[1]
- pour laver les puits d'une plaque de microtitration[2].
- pour laver des cellules avant de les remettre en suspension dans un tampon de lyse pour isoler de l'ADN[3].
- comme constituant d'un milieu de conservation pour embryons destiné à la vitrification des embryons.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Eu gostaria de saber a osmolaridade da solução de DMPBS.
1 answer-
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Quería saber si éste producto se puede considerar RNAsa free para realizar durante la hibridación in situ
1 answer-
This product is a sterile-filtered M Quality Level 300 product. It is not molecular biology grade and does not have specifications such as RNase and DNase free. Therefore, this product cannot be considered RNase free for use in in situ hybridization.
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Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide
1 answer-
This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.
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Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?
1 answer-
This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.
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What is the concentration of the product in Molarity?
1 answer-
This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
Product D8537 contains:
Potassium chloride: 0.2 g/l
Potassium phosphate monobasic: 0.2 g/l
Sodium chloride: 8 g/l
Sodium Phosphate dibasic: 1.15 g/lPlease see the link below for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdfHelpful?
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What is the bottle thread type on the 1L bottle (D8537-1L)
1 answer-
The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
Thread Type 38-430
Thread Diameter 37.3 mmHelpful?
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What is the pH of a solution of Product D8537, Dulbecco's Phosphate?
1 answer-
The pH will be in the range of 7.1-7.5, according to the specification sheet.
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Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?
1 answer-
This is a 1X concentration. The 10X concentration is listed under product D1408.
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What is a Salt Toxicity Test?
1 answer-
The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted. They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour. The cells are counted and %viability determined. The cells are then cultured for an additional 7 hours (total of 8 hours). The cells are counted again and the % viability determined. The viability at 1 hr has to be >95% of the original viability. The viability at 8 hrs must be >75% of the original viability.
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What is the formulation for Product D8537, Dulbecco's Phosphate?
1 answer-
The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.
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